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Insulin Degrading Enzyme (9B12.225) antibody immnunostaining on a paraffin embedded section of human brain. Intense IDE staining is observed with ab25733 in hippocampal dentate granular layer, hilus and CA-2/3 with lower low levels in the CA1 region.
S Craft, VAPSHCS, United States
All lanes : Anti-Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733) at 1/1000 dilution
Lane 1 : Murine embryonic fibroblast lysate at 20 µg
Lane 2 : Murine embryonic fibroblast lysate at 20 µg
Lane 3 : HeLa cell lysate at 40 µg
Lane 4 : HeLa cell lysate at 20 µg
Secondary
HRP conjugated rabbit anti-mouse IgG at 1/10000 dilution
Predicted band size : 110 kDa
Observed band size : 118 kDa (why is the actual band size different from the predicted?)
Image courtesy of Dr Gillian Hamilton by Abreview.
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