![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733) image](#ab25733_1.jpg)
![Western blot - Anti-Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733) image](#Insulin-degrading-enzyme-IDE-Primary-antibodies-ab25733-21.jpg)
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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Insulin degrading enzyme / IDE antibody [9B12.225]
See all Insulin degrading enzyme / IDE products (11) ...
Description
Mouse monoclonal [9B12.225] to Insulin degrading enzyme / IDE
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Hamster, Human, Monkey
Does not react with
Mouse
Immunogen
purified human erythrocyte IDE.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
9B12.225
Isotype
IgG1
Light chain type
kappa
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> Insulysin
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP
IP: 1/150IP: 1/150
IHC-P: Use at an assay dependent dilution.
Target
Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similarities
Belongs to the peptidase M16 family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Target information above from: UniProt accessionP14735
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Database links
- Entrez Gene: 3416 Human
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: P14735 Human
- SwissProt: Q5T5N2 Human
- SwissProt: P35559 Rat
- Unigene: 500546 Human
- Unigene: 45029 Rat
Anti-Insulin degrading enzyme / IDE antibody [9B12.225] images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733)
![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733)](/ps/datasheet/Images/25/ab25733/ab25733_1.jpg)
Insulin Degrading Enzyme (9B12.225) antibody immnunostaining on a paraffin embedded section of human brain. Intense IDE staining is observed with ab25733 in hippocampal dentate granular layer, hilus and CA-2/3 with lower low levels in the CA1 region.
S Craft, VAPSHCS, United States
Western blot - Anti-Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733)
![Western blot - Anti-Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733)](/ps/datasheet/images/25/ab25733/Insulin-degrading-enzyme-IDE-Primary-antibodies-ab25733-21.jpg)
All lanes : Anti-Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733) at 1/1000 dilution
Lane 1 : Murine embryonic fibroblast lysate at 20 µg
Lane 2 : Murine embryonic fibroblast lysate at 20 µg
Lane 3 : HeLa cell lysate at 40 µg
Lane 4 : HeLa cell lysate at 20 µg
Secondary
HRP conjugated rabbit anti-mouse IgG at 1/10000 dilution
Predicted band size : 110 kDa
Observed band size : 118 kDa (why is the actual band size different from the predicted?)
Image courtesy of Dr Gillian Hamilton by Abreview.
References for Anti-Insulin degrading enzyme / IDE antibody [9B12.225] (ab25733)
This product has been referenced in:
- Kilger Eet al. BRI2 Protein Regulates {beta}-Amyloid Degradation by Increasing Levels of Secreted Insulin-degrading Enzyme (IDE). J Biol Chem 286:37446-57 (2011). IP; Human.Read more (PubMed: 21873424) »
- Solas Met al. Interactions between age, stress and insulin on cognition: implications for Alzheimer's disease. Neuropsychopharmacology 35:1664-73 (2010). WB; Rat.Read more (PubMed: 20182419) »
See all 6 publications for this product
Publishing research using ab25733? Please let us know so that we can cite the reference in this datasheet
![Insulin degrading enzyme / IDE antibody [9B12.225] for Western blot in Human (25733)](/ps/datasheet/images/25/ab25733/Insulin-degrading-enzyme-IDE-Primary-antibodies-ab25733-19.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"