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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Insulin degrading enzyme / IDE antibody - Mid molecule
See all Insulin degrading enzyme / IDE products (11) ...
Description
Rabbit polyclonal to Insulin degrading enzyme / IDE - Mid molecule
Specificity
ab28562 recognises the middle region of IDE.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the middle region of human Insulin degrading enzyme / IDE. (Peptide available as ab41260.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> Insulysin
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Western blot - Insulin degrading enzyme / IDE antibody (ab28562)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 118 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similarities
Belongs to the peptidase M16 family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Target information above from: UniProt accessionP14735
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Database links
- Entrez Gene: 3416 Human
- Omim: 146680 Human
- SwissProt: P14735 Human
- SwissProt: Q5T5N2 Human
- Unigene: 500546 Human
Anti-Insulin degrading enzyme / IDE antibody - Mid molecule images:
Western blot - Insulin degrading enzyme / IDE antibody (ab28562)
All lanes : Anti-Insulin degrading enzyme / IDE antibody - Mid molecule (ab28562) at 1/1000 dilution
Lane 1 : Insulysin at 0.05 µg
Lane 2 : Insulysin at 0.01 µg
Lane 3 : Insulysin at 0.001 µg
Predicted band size : 118 kDa
Observed band size : 113 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Insulin degrading enzyme / IDE antibody - Mid molecule (ab28562)
ab28562 has not yet been referenced specifically in any publications.
Publishing research using ab28562? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"