Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab25970 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Hello, I am using your anti-IDE product (ab25970) in my lab. I was looking for the stock concentration of your product, but I couldn't find it neither on the product nor in your website. I would appreciate it if you could let me know of the concentration, or refer me to a document that specifies the data. Kind regards. |
|||||||||
ANSWER: |
Thank you for your inquiry. The Anti-Insulin degrading enzyme / IDE antibody ab25970 is provided as whole antiserum. Such unpurified antibodies will not have a concentration stated on the datasheet, as for most whole antiserum, culture supernatant or ascites fluid products the concentration has not been determined. This is because unpurified antibody preparations vary significantly their protein concentration and only a fraction of the proteins is the specific antibody. As the specific antibody concentration is unknown, you may refer to the “concentration estimates” (attached) as a guideline for estimation. Please remember that these dilutions and concentration estimates are simply recommended as a starting point, and it may be necessary to adjust the dilution based on the experimental results. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab25970) at 1/1000 dilution
Lane 1 : Hippocampus (Mouse) Tissue Lysate
Lane 2 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 118 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
0
Call 01223 696 000 or contact us