Insulin degrading enzyme / IDE antibody (ab25970)

Product Name 

Insulin degrading enzyme / IDE antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Insulin degrading enzyme / IDE

Immunogen 

Recombinant fusion protein fragment: IGSLSDPP N IAGLSHFCE HMLFLGTKKY PKENEYSQF L SEHAGSSN AF TSGEHTN YYF DVSHEH LEGA LDRFA QFFLC PLFD ESCKDR EVN AVDSEHE KN VMNDAWRL F QLEKATGNP KHPFSKFGTG NKYTLETRP N QEGIDVRQEL LKFHSAYYSS NLMAVCVLGR ESLDDLTNLV VKL , corresponding to amino acids 93-273 of Rat Insulin degrading enzyme / IDE
BLAST 'IGSLSDPP N IAGLSHFCE HMLFLGTKKY PKENEYSQF L SEHAGSSN AF TSGEHTN YYF DVSHEH LEGA LDRFA QFFLC PLFD ESCKDR EVN AVDSEHE KN VMNDAWRL F QLEKATGNP KHPFSKFGTG NKYTLETRP N QEGIDVRQ ' with or

Reacts with 
(species key)

Hu, Ms, Rat, Hm

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/2000 - 1/4000.
ICC: 1/200 - 1/1000.
WB: 1/500 - 1/4000. Predicted molecular weight: 118 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> Insulysin
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer’s disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases.

Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: Whole serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

References for Insulin degrading enzyme / IDE antibody (ab25970)

  This product has been used in: (two most recent references)

Hiltunen M et al. Focal cerebral ischemia in rats alters APP processing and expression of Abeta peptide degrading enzymes in the thalamus. Neurobiol Dis 35:103-13 (2009). WB; Rat. PubMed: 19426802

Wang J et al. Grape-derived polyphenolics prevent Abeta oligomerization and attenuate cognitive deterioration in a mouse model of Alzheimer's disease. J Neurosci 28:6388-92 (2008). PubMed: 18562609

See all 3 publication references for this product.


If you publish research using ab25970 please let us know so that we can cite the reference on this datasheet.

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