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Anti-Insulin degrading enzyme / IDE antibody (ab25970)

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Overview

Product name

Anti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE products (11) ...

Description

Rabbit polyclonal to Insulin degrading enzyme / IDE

Tested applications

ELISA, WB, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Human

Immunogen

Recombinant fusion protein fragment: IGSLSDPP N IAGLSHFCE HMLFLGTKKY PKENEYSQF L SEHAGSSN AF TSGEHTN YYF DVSHEH LEGA LDRFA QFFLC PLFD ESCKDR EVN AVDSEHE KN VMNDAWRL F QLEKATGNP KHPFSKFGTG NKYTLETRP N QEGIDVRQEL LKFHSAYYSS NLMAVCVLGR ESLDDLTNLV VKL , corresponding to amino acids 93-273 of Rat Insulin degrading enzyme / IDE

IGSLSDPP NIAGLSHFCE HMLFLGTKKY PKENEYSQF L SEHAGSSNAF TSGEHTNYYF DVSHEHLEGA LDRFA QFFLC PLFDESCKDR EVNAVDSEHE KNVMNDAWRL F QLEKATGNP KHPFSKFGTG NKYTLETRPN QEGIDVRQ EL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> Insulysin
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

  • Western blot - Insulin degrading enzyme / IDE antibody (ab25970)Western blot - Insulin degrading enzyme / IDE antibody (ab25970) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/2000 - 1/4000.

    ELISA: 1/2000 - 1/4000.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/4000.Predi...Read more →

    WB: 1/500 - 1/4000.Predicted molecular weight: 118 kDa.

  • ICC

     ICC: 1/200 - 1/1000.

    ICC: 1/200 - 1/1000.

Target

Function

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

Sequence similarities

Belongs to the peptidase M16 family.

Post-translational
modifications

The N-terminus is blocked.

Cellular localization

Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

Target information above from: UniProt accessionP14735 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Abeta-degrading protease antibody
  • FLJ35968 antibody
  • Ide antibody
  • IDE_HUMAN antibody
  • insulin protease antibody
  • Insulin-degrading enzyme antibody
  • insulinase antibody
  • insulysin antibody
  • OTTHUMP00000020097 antibody
see all

Database links

see all

Anti-Insulin degrading enzyme / IDE antibody images:

  Western blot - Insulin degrading enzyme / IDE antibody (ab25970)

Western blot - Insulin degrading enzyme / IDE antibody (ab25970)

All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab25970) at 1/1000 dilution

Lane 1 : Hippocampus (Mouse) Tissue Lysate
Lane 2 : Rat Hippocampus Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 118 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 90 seconds

References for Anti-Insulin degrading enzyme / IDE antibody (ab25970)

This product has been referenced in:

  • Glebov Ket al. Functional relevance of a novel SlyX motif in non-conventional secretion of insulin-degrading enzyme. J Biol Chem 286:22711-5 (2011). WB.Read more (PubMed: 21576244) »
  • Kilger Eet al. BRI2 Protein Regulates {beta}-Amyloid Degradation by Increasing Levels of Secreted Insulin-degrading Enzyme (IDE). J Biol Chem 286:37446-57 (2011). WB; Human.Read more (PubMed: 21873424) »

See all 7 publications for this product

Publishing research using ab25970? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"