Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE products (11) ...
Description
Rabbit polyclonal to Insulin degrading enzyme / IDE
Tested applications
WB, IHC-P, IHC-FoFr, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Cat, Dog
Immunogen
Synthetic peptide derived from within residues 950 to the C-terminus of Human Insulin degrading enzyme / IDE.
(Peptide available as ab32215.)
Positive control
Mouse and Rat brain lysate. Mouse and Rat hippocampus lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> Insulysin
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
-
Western blot - Insulin degrading enzyme / IDE antibody (ab32216)
(enlarge)
-
Immunohistochemistry - Free Floating - Insulin degrading enzyme / IDE antibody (ab32216)
(enlarge)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin degrading enzyme / IDE antibody (ab32216)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
IHC-P: 1/50Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr: 1/100
IHC-Fr: 1/500
Target
Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similarities
Belongs to the peptidase M16 family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Target information above from: UniProt accessionP14735
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Database links
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: P14735 Human
- SwissProt: Q5T5N2 Human
- SwissProt: Q8CGB9 Mouse
- SwissProt: Q9JHR7 Mouse
see all
Anti-Insulin degrading enzyme / IDE antibody images:
Western blot - Insulin degrading enzyme / IDE antibody (ab32216)
All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/ml
Lane 1 : Mouse Brain at 20 µg/ml
Lane 2 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3 : Mouse Hippocampus Lysate at 20 µg
Lane 4 : Rat Hippocampus Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 118 kDa
Observed band size : 118 kDa
Immunohistochemistry - Free Floating - Insulin degrading enzyme / IDE antibody (ab32216)
Immunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
Sophie Pezet,CNRS, Paris
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin degrading enzyme / IDE antibody (ab32216)
Image courtesy of Human Protein Atlas
ab32216 staining human Gall bladder. The paraffin embedded human skin tissue was incubated with ab32216 (1/50 dilution) for 30 mins at room temperature. The staining is predominantly cytoplasmic although some nuclear staining can be seen. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab32216 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
References for Anti-Insulin degrading enzyme / IDE antibody (ab32216)
This product has been referenced in:
- Niikura Tet al. A humanin derivative reduces amyloid Beta accumulation and ameliorates memory deficit in triple transgenic mice. PLoS One 6:e16259 (2011). IHC-Fr; Mouse.Read more (PubMed: 21264226) »
- Griffiths HHet al. Prion Protein Interacts with BACE1 Protein and Differentially Regulates Its Activity toward Wild Type and Swedish Mutant Amyloid Precursor Protein. J Biol Chem 286:33489-500 (2011). WB; Human.Read more (PubMed: 21795680) »
See all 4 publications for this product
Publishing research using ab32216? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"