![Immunocytochemistry/ Immunofluorescence - Integrin alpha 2+beta 1 antibody [16B4] (ab30483) image](#Integrin-alpha-2beta-1-Primary-antibodies-ab30483-2.jpg)
![Immunocytochemistry/ Immunofluorescence - Integrin alpha 2+beta 1 antibody [16B4] (ab30483) image](#ab30483_1.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-Integrin alpha 2+beta 1 antibody [16B4]
See all Integrin alpha 2+beta 1 products (3) ...
Description
Mouse monoclonal [16B4] to Integrin alpha 2+beta 1
Specificity
We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
ICC/IF, IHC-P, IP, WB, Flow Cyt, ELISA, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
Positive control
In IF, this antibody gave a positive signal in HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
ab30483 was purified from tissue culture supernatant
Primary antibody notes
ab30483 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
Clonality
Monoclonal
Clone number
16B4
Myeloma
x63-Ag8.653
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(Tris/EDTA pH 9.0 is recommended for this purpose.)
IP: Use a concentration of 25 - 50 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 131 kDa.
Flow Cyt: Use a concentration of 30 µg/ml
ELISA: Use a concentration of 10 µg/ml
IHC-Fr: Use a concentration of 30 - 40 µg/ml.
Target
Relevance
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells.
Cellular localization
Cell Membrane; single-pass type I membrane protein
Alternative names
- BR antibody
- CD29 antibody
- CD29 antigen antibody
see all
Database links
- Entrez Gene: 3673 Human
- Entrez Gene: 3688 Human
- Omim: 135630 Human
- Omim: 192974 Human
- SwissProt: P05556 Human
- SwissProt: P17301 Human
- Unigene: 482077 Human
- Unigene: 643813 Human
Anti-Integrin alpha 2+beta 1 antibody [16B4] images:
Immunocytochemistry/ Immunofluorescence - Integrin alpha 2+beta 1 antibody [16B4] (ab30483)
![Immunocytochemistry/ Immunofluorescence - Integrin alpha 2+beta 1 antibody [16B4] (ab30483)](/ps/datasheet/images/30/ab30483/Integrin-alpha-2beta-1-Primary-antibodies-ab30483-2.jpg)
ICC/IF image of ab30483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.
Immunocytochemistry/ Immunofluorescence - Integrin alpha 2+beta 1 antibody [16B4] (ab30483)
![Immunocytochemistry/ Immunofluorescence - Integrin alpha 2+beta 1 antibody [16B4] (ab30483)](/ps/datasheet/Images/30/ab30483/ab30483_1.jpg)
ab30483 staining human breast cancer cells by ICC/IF. Cells were PFA fixed and blocked with 1% serum for 16 hours at 20°C prior to incubating with ab30483 (at 30µg/ml) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Sharon Sneddon
References for Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483)
This product has been referenced in:
- Tuckwell DSet al. Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a functionally important region within alpha2beta1. Biochem J 350 Pt 2:485-93 (2000).Read more (PubMed: 10947963) »
- Fitter Set al. Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion. Biochem J 338 ( Pt 1):61-70 (1999).Read more (PubMed: 9931299) »
See all 3 publications for this product
Publishing research using ab30483? Please let us know so that we can cite the reference in this datasheet
![Integrin alpha 2 beta 1 antibody [16B4] for ICC/IF in Human (30483)](/ps/Reviews/Images/ab11403_59587.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"