Integrin alpha 2+beta 1 antibody [16B4] (ab30483)

Product Name 

Integrin alpha 2+beta 1 antibody [16B4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [16B4] to Integrin alpha 2+beta 1

Immunogen 

Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.

Reacts with 

Hu

Does not react with 
(species key)

Rat

Tested applications 
(see key)

ELISA, Flow Cyt, ICC/IF, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 10 µg/ml.
Flow Cyt: Use at a concentration of 30 µg/ml.
ICC/IF: Use at a concentration of 30 - 40 µg/ml.
IHC-Fr: Use at a concentration of 30 - 40 µg/ml.
IP: Use at a concentration of 25 - 50 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 131 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane; single-pass type I membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

Relevance 

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells.

ab30483 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I¹²⁵ surface labelled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

16B4

Myeloma 

x63-Ag8.653

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab30483 was purified from tissue culture supernatant

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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References for Integrin alpha 2+beta 1 antibody [16B4] (ab30483)

  This product has been used in: (two most recent references)

Fitter S et al. Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion. Biochem J 338 ( Pt 1):61-70 (1999). PubMed: 9931299

Sincock PM et al. PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function. J Cell Sci 112 ( Pt 6):833-44 (1999). PubMed: 10036233

See all 3 publication references for this product.


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