Integrin alpha 2+beta 1 antibody [P1E6] (ab24697)

Product Name 

Integrin alpha 2+beta 1 antibody [P1E6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [P1E6] to Integrin alpha 2+beta 1

Immunogen 

Tissue / cell preparation (Human): HT1080 fibrosarcoma cells.

Reacts with 
(species key)

Hu

Specificity 

ab24697 is directed against the a2 subunit and will precipitate a complex containing a2, including a2ß1 Integrin and neutralizes binding to collagen.

Tested applications 
(see key)

BL, Flow Cyt, ICC/IF, IP, Inhib, Neut

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
BL: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution (PMID 19780039).
IF: 1/1000.
IP: Use at an assay dependent dilution.
Inhib: Use at an assay dependent dilution.
Neut: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane; single-pass type I membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

Relevance 

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells.

ab24697 is effective in blocking adhesion cells to collagen coated surfaces. It immunoprecipitates the a2 subunit (reducing) or a2 complexed with ß1 from surface labeled cell lysates (non-reducing). In imunofluorescence, this antibody can be used to detect a2 in collagen dependent focal adhesions.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

P1E6

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: None
Constituents: Ascites

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for Integrin alpha 2+beta 1 antibody [P1E6] (ab24697)

  This product has been used in: (two most recent references)

Lindberg K et al. Expression of estrogen receptor beta increases integrin alpha1 and integrin beta1 levels and enhances adhesion of breast cancer cells. J Cell Physiol 222:156-67 (2010). Flow Cyt; Human. PubMed: 19780039

Bailey CL et al. Activation of Rap1 promotes prostate cancer metastasis. Cancer Res 69:4962-8 (2009). Inhib; Human. PubMed: 19470770

See all 3 publication references for this product.


If you publish research using ab24697 please let us know so that we can cite the reference on this datasheet.

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