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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-Integrin alpha 2+beta 1 antibody [P1E6]
See all Integrin alpha 2+beta 1 products (3) ...
Description
Mouse monoclonal [P1E6] to Integrin alpha 2+beta 1
Specificity
ab24697 is directed against the a2 subunit and will precipitate a complex containing a2, including a2ß1 Integrin and neutralizes binding to collagen.
Tested applications
IF, IP, Blocking, Neutralising, Flow Cyt, Inhibition Assay, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human): HT1080 fibrosarcoma cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
ab24697 is effective in blocking adhesion cells to collagen coated surfaces. It immunoprecipitates the a2 subunit (reducing) or a2 complexed with ß1 from surface labeled cell lysates (non-reducing). In imunofluorescence, this antibody can be used to detect a2 in collagen dependent focal adhesions.
Clonality
Monoclonal
Clone number
P1E6
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
IF: 1/1000IF: 1/1000
IP: Use at an assay dependent dilution.
BL: Use at an assay dependent dilution.
Neut: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution. (PubMed: 19780039)
Inhib: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution. (PubMed: 20533192)
Target
Relevance
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells.
Cellular localization
Cell Membrane; single-pass type I membrane protein
Alternative names
- BR antibody
- CD29 antibody
- CD29 antigen antibody
see all
Database links
- Entrez Gene: 3673 Human
- Entrez Gene: 3688 Human
- Omim: 135630 Human
- Omim: 192974 Human
- SwissProt: P05556 Human
- SwissProt: P17301 Human
- Unigene: 482077 Human
- Unigene: 643813 Human
References for Anti-Integrin alpha 2+beta 1 antibody [P1E6] (ab24697)
This product has been referenced in:
- Lindberg Ket al. Expression of estrogen receptor beta increases integrin alpha1 and integrin beta1 levels and enhances adhesion of breast cancer cells. J Cell Physiol 222:156-67 (2010). Flow Cyt; Human.Read more (PubMed: 19780039) »
- Coelho NMet al. Different assembly of type IV collagen on hydrophilic and hydrophobic substrata alters endothelial cells interaction. Eur Cell Mater 19:262-72 (2010). ICC/IF; Human.Read more (PubMed: 20533192) »
See all 4 publications for this product
Publishing research using ab24697? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"