![Flow Cytometry - Integrin alpha 4 antibody [44H6] (FITC) (ab221) image](#ab221_1.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-Integrin alpha 4 antibody [44H6] (FITC)
See all Integrin alpha 4 products (32) ...
Description
Mouse monoclonal [44H6] to Integrin alpha 4 (FITC)
Conjugation
FITC
Conjugation notes
Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.
Specificity
44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation.
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
HOON pre-B leukaemia cell line
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified IgG prepared by ion exchange chromotography from tissue culture supernatant.
Clonality
Monoclonal
Clone number
44H6
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Research areas
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry - Integrin alpha 4 antibody [44H6] (FITC) (ab221)
![Flow Cytometry - Integrin alpha 4 antibody [44H6] (FITC) (ab221) image](/ps/datasheet/Images/0/ab221/ab221_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 106 cells. (or 100µl whole blood.)
Target
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
Post-translational
modifications
Phosphorylation on Ser-1027 inhibits PXN binding.
Cellular localization
Membrane.
Target information above from: UniProt accessionP13612
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 269C wild type antibody
- Alpha 4 subunit of VLA 4 receptor antibody
- Antigen CD49D antibody
see all
Database links
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
Anti-Integrin alpha 4 antibody [44H6] (FITC) images:
Flow Cytometry - Integrin alpha 4 antibody [44H6] (FITC) (ab221)
ab221 staining Integrin alpha 4 (FITC) in human peripheral blood lymphocytes by Flow Cytometry analysis.
References for Anti-Integrin alpha 4 antibody [44H6] (FITC) (ab221)
ab221 has not yet been referenced specifically in any publications.
Publishing research using ab221? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"