![Flow Cytometry / FACS - Integrin alpha 4 antibody [44H6] (ab220) image](#ab220_1.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-Integrin alpha 4 antibody [44H6]
See all Integrin alpha 4 products (32) ...
Description
Mouse monoclonal [44H6] to Integrin alpha 4
Specificity
Recognises the integrin alpha 4 subunit, which associates with either Integrin beta 1 (to form VLA 4) or beta 7. The alpha 4 integrin is a 150 kDa molecule, which may be cleaved to 80 kDa and 70 kDa fragments.
Tested applications
Flow Cyt, IHC-Fr, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
HOON pre-B leukaemia cell line.
Positive control
FACS: human whole peripheral blood IHC-Fr: Human Tonsil
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified IgG prepared by ion exchange chromotography from tissue culture supernatant.
Clonality
Monoclonal
Clone number
44H6
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Research areas
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry / FACS - Integrin alpha 4 antibody [44H6] (ab220)
![Flow Cytometry / FACS - Integrin alpha 4 antibody [44H6] (ab220) image](/ps/datasheet/Images/0/ab220/ab220_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/40(Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.)
IHC-Fr
IHC-Fr: 1/50 - 1/100.IHC-Fr: 1/50 - 1/100.
WB: 1/200
IP
IP: 1/20 - 1/25.IP: 1/20 - 1/25.
Application notes
Is unsuitable for or IHC-P.
Target
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
Post-translational
modifications
Phosphorylation on Ser-1027 inhibits PXN binding.
Cellular localization
Membrane.
Target information above from: UniProt accessionP13612
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 269C wild type antibody
- Alpha 4 subunit of VLA 4 receptor antibody
- Antigen CD49D antibody
see all
Database links
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
Anti-Integrin alpha 4 antibody [44H6] images:
Flow Cytometry / FACS - Integrin alpha 4 antibody [44H6] (ab220)
Staining of human peripheral blood lymphocytes with ab220.
References for Anti-Integrin alpha 4 antibody [44H6] (ab220)
This product has been referenced in:
- Jouhilahti EMet al. The development of cutaneous neurofibromas. Am J Pathol 178:500-5 (2011).Read more (PubMed: 21281783) »
See 1 publication for this product
Publishing research using ab220? Please let us know so that we can cite the reference in this datasheet
![Integrin alpha 4 antibody [44H6] for WB in Human (220)](/ps/Reviews/Images/ab12967_73360.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"