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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-Integrin alpha 4 antibody [HP2/1]
See all Integrin alpha 4 products (32) ...
Description
Mouse monoclonal [HP2/1] to Integrin alpha 4
Specificity
This antibody recognises the 80kD alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells.
Tested applications
Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Rat, Cow, Human, Pig, Rhesus monkey
Immunogen
JM leukaemia line (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.
Clonality
Monoclonal
Clone number
HP2/1
Myeloma
x63-Ag8.653
Isotype
IgG1
Research areas
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Immunology >> Cell Type Markers >> CD >> Non-lineage
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 105 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Target
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
Post-translational
modifications
Phosphorylation on Ser-1027 inhibits PXN binding.
Cellular localization
Membrane.
Target information above from: UniProt accessionP13612
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 269C wild type antibody
- Alpha 4 subunit of VLA 4 receptor antibody
- Antigen CD49D antibody
see all
Database links
- Entrez Gene: 3676 Human
- Entrez Gene: 311144 Rat
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
- Unigene: 12704 Rat
References for Anti-Integrin alpha 4 antibody [HP2/1] (ab22858)
ab22858 has not yet been referenced specifically in any publications.
Publishing research using ab22858? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"