![Flow Cytometry / FACS - Integrin alpha 4 antibody [PS/2] (ab24944) image](#ab24944_FACS.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-Integrin alpha 4 antibody [PS/2] (Biotin)
See all Integrin alpha 4 products (32) ...
Description
Rat monoclonal [PS/2] to Integrin alpha 4 (Biotin)
Conjugation
Biotin
Specificity
ab24944 recognises Integrin alpha 4.
Tested applications
Functional Studies, Flow Cyt, IP, Inhibition Assaymore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
The details of the immunogen for this antibody are not available.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
PS/2
Isotype
IgG2b
Light chain type
kappa
Research areas
Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry / FACS - Integrin alpha 4 antibody [PS/2] (ab24944)
![Flow Cytometry / FACS - Integrin alpha 4 antibody [PS/2] (ab24944) image](/ps/datasheet/Images/24/ab24944/ab24944_FACS.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution. ((Studies of cell-cell interaction and tumor cell metastasis and adhesion; Retardation of myelopoiesis in Dexter-type cultures).)
Flow Cyt: Use 2µl for 106 cells.
IP: Use at an assay dependent dilution.
Inhib: Use at an assay dependent dilution. ((Inhibition of lymphopoiesis in Whitlock-Witte long-term bone marrow cultures))
Target
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
Post-translational
modifications
Phosphorylation on Ser-1027 inhibits PXN binding.
Cellular localization
Membrane.
Target information above from: UniProt accessionP13612
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 269C wild type antibody
- Alpha 4 subunit of VLA 4 receptor antibody
- Alpha 4 subunit of VLA 4 receptor antibody
see all
Database links
- Entrez Gene: 3676 Human
- Entrez Gene: 16401 Mouse
- Omim: 192975 Human
- SwissProt: P13612 Human
- SwissProt: Q00651 Mouse
- Unigene: 440955 Human
- Unigene: 31903 Mouse
Anti-Integrin alpha 4 antibody [PS/2] (Biotin) images:
References for Anti-Integrin alpha 4 antibody [PS/2] (Biotin) (ab24944)
ab24944 has not yet been referenced specifically in any publications.
Publishing research using ab24944? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"