![Western blot - Integrin alpha 5 antibody [10F6] (ab93943) image](#Integrin-alpha-5-Primary-antibodies-ab93943-1.jpg)
![Flow Cytometry - Integrin alpha 5 antibody [10F6] (ab93943) image](#Integrin-alpha-5-Primary-antibodies-ab93943-2.jpg)
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Product name
Anti-Integrin alpha 5 antibody [10F6]
See all Integrin alpha 5 products (20) ...
Description
Mouse monoclonal [10F6] to Integrin alpha 5
Tested applications
Flow Cyt, WB, ELISA, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified recombinant fragment of Human Integrin alpha 5 expressed in E. coli.
Positive control
WB: Integrin alpha 5-hIgGFc transfected HEK293 cell lysate Flow Cyt: HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
10F6
Isotype
IgG2a
Research areas
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab93943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/10,000.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: 1/200 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IHC-Fr: 1/200 - 1/1000.
WB: 1/500 - 1/2000. Predicted molecular weight: 115 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
see all
Database links
- Entrez Gene: 3678 Human
- Omim: 135620 Human
- SwissProt: P08648 Human
- Unigene: 505654 Human
Anti-Integrin alpha 5 antibody [10F6] images:
Western blot - Integrin alpha 5 antibody [10F6] (ab93943)
![Western blot - Integrin alpha 5 antibody [10F6] (ab93943)](/ps/datasheet/images/93/ab93943/Integrin-alpha-5-Primary-antibodies-ab93943-1.jpg)
Anti-Integrin alpha 5 antibody [10F6] (ab93943) at 1/500 dilution + Integrin alpha 5-hIgGFc transfected HEK293 cell lysate.
Predicted band size : 115 kDa
Flow Cytometry - Integrin alpha 5 antibody [10F6] (ab93943)
![Flow Cytometry - Integrin alpha 5 antibody [10F6] (ab93943)](/ps/datasheet/images/93/ab93943/Integrin-alpha-5-Primary-antibodies-ab93943-2.jpg)
Flow cytometric analysis of Hela cells using ab93943 (green) and a negative control (purple).
References for Anti-Integrin alpha 5 antibody [10F6] (ab93943)
ab93943 has not yet been referenced specifically in any publications.
Publishing research using ab93943? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"