![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251) image](#Integrin-alpha-5-Primary-antibodies-ab25251-29.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251)
Overview
Product name
Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free
See all Integrin alpha 5 products (19) ...
Description
Rat monoclonal [5H10-27 (MFR5)] to Integrin alpha 5 - BSA and Azide free
Specificity
ab25251 is specific for the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
Tested applications
ICC/IF, Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: None
Constituents: 100mM Borate buffered saline, pH 8.2
Concentration
Concentration information loading...
Primary antibody notes
Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.
Clonality
Monoclonal
Clone number
5H10-27 (MFR5)
Isotype
IgG2a
Light chain type
kappa
Research areas
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
-
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251)
![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251) image](/ps/datasheet/images/25/ab25251/Integrin-alpha-5-Primary-antibodies-ab25251-29.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
IHC-Fr
Application notes
Flow Cyt: Use at an assay dependent dilution. Use up to 1ug/106 cells.
IHC-Fr: Use at an assay dependent dilution.
T-cell co-stimulation: Use at an assay dependent dilution.
In vitro blocking of adhesion: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha 5 beta 1 antibody
- CD49 antigen-like family member E antibody
- CD49e antibody
see all
Database links
- Entrez Gene: 16402 Mouse
- SwissProt: P11688 Mouse
- Unigene: 16234 Mouse
Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free images:
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251)
ab25251 staining Integrin alpha 5 in Mouse Embryonic Fibroblast (MEF) cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in Triton X-100. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 546-conjugated goat anti-rat polyclonal, diluted 1/200.
This image is courtesy of an Abreview submitted by Benjamin Geiger
References for Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251)
This product has been referenced in:
- Rich Set al. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol 157:2916-23 (1996).Read more (PubMed: 8816397) »
- Uhlenkott CEet al. Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine. Clin Exp Metastasis 14:125-37 (1996).Read more (PubMed: 8605726) »
See all 5 publications for this product
Publishing research using ab25251? Please let us know so that we can cite the reference in this datasheet
![Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free for Immunocytochemistry/ Immunofluorescence in Mouse (25251)](/ps/datasheet/images/25/ab25251/Integrin-alpha-5-Primary-antibodies-ab25251-21.jpg)
![Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free for Immunocytochemistry/ Immunofluorescence in Human (25251)](/ps/datasheet/images/25/ab25251/Integrin-alpha-5-Primary-antibodies-ab25251-27.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"