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Product Name
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Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free
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See all Integrin alpha 5 antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [5H10-27 (MFR5)] to Integrin alpha 5 - BSA and Azide free
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Immunogen
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C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)
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Reacts with
(species key)
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Ms
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Specificity
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ab25251 is specific for the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
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Tested applications
(see key)
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Flow Cyt, IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent dilution. Use up to 1ug/106 cells.
IHC-Fr: Use at an assay dependent dilution.
T-cell co-stimulation: Use at an assay dependent dilution.
In vitro blocking of adhesion: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Relevance
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Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling.
Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.
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Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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5H10-27 (MFR5)
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Isotype
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IgG2a
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Light chain type
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kappa
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Storage buffer
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Preservative: No preservatives or amine-containing buffer salts added.
Constituents: 100mM Borate. pH 8.0
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free (ab25251)
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This clone has been used in: (two most recent references)
Rich S et al. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol 157:2916-23 (1996). PubMed: 8816397
Uhlenkott CE et al. Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine. Clin Exp Metastasis 14:125-37 (1996). PubMed: 8605726
See all 5 publication references for this product.
If you publish research using ab25251 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Integrin alpha 5
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Integrin alpha 5 antibody [5H10-27 (MFR5)] - BSA and Azide free - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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