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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-Integrin alpha 5 antibody [5H10-27] (Phycoerythrin)
See all Integrin alpha 5 products (19) ...
Description
Rat monoclonal [5H10-27] to Integrin alpha 5 (Phycoerythrin)
Conjugation
Phycoerythrin
Specificity
ab25461 recognises CD49e, the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
Tested applications
ICC/IF, Flow Cyt, IHC-Fr, Blocking, Cellular activationmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Tissue / cell preparation: C57BL/6 x A/J F1 mouse mast cell line MC/9
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, PBS; Stabilizing agent
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
5H10-27
Isotype
IgG2a
Light chain type
kappa
Research areas
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
IHC-Fr
Blocking
Cellular activation
Application notes
BL: Use at an assay dependent dilution.
Note: ab25461 can be used for in vitro blocking of adhesion.
Cellular activation: Use at an assay dependent dilution.
Note: ab25461 can be used for T cell co-stimulation
Flow Cyt: Use 0.3µg for 106 cells.
ICC/IF: 1/150.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha 5 beta 1 antibody
- CD49 antigen-like family member E antibody
- CD49e antibody
see all
Database links
- Entrez Gene: 16402 Mouse
- SwissProt: P11688 Mouse
- Unigene: 16234 Mouse
References for Anti-Integrin alpha 5 antibody [5H10-27] (Phycoerythrin) (ab25461)
ab25461 has not yet been referenced specifically in any publications.
Publishing research using ab25461? Please let us know so that we can cite the reference in this datasheet
![Integrin alpha 5 antibody [5H10-27] (Phycoerythrin) for ICC/IF in Mouse (25461)](/ps/Reviews/Images/ab8659_38046.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"