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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-Integrin alpha 5 antibody [JBS5]
See all Integrin alpha 5 products (19) ...
Description
Mouse monoclonal [JBS5] to Integrin alpha 5
Tested applications
ICC/IF, Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Dog, Human
Immunogen
The details of the immunogen for this antibody are not available.
Positive control
Recommended positive control tissue: Human Endothelium (IHC-Fr)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
This antibody reacts with the Alpha 5 integrin. Has been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines. It also inhibits the adherence of cells to fibronectin.
Clonality
Monoclonal
Clone number
JBS5
Myeloma
NS1
Isotype
IgG
Research areas
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
Flow Cyt
IHC-Fr
IP
Application notes
Flow Cyt: Use 1µg for 106 cells.
ICC/IF: Use at an assay dependent dilution (PMID 19695696).
IHC-Fr: Use at a concentration of 0.5 - 1 µg/ml.
IP: Use at a concentration of 1 - 5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha 5 beta 1 antibody
- CD49 antigen-like family member E antibody
- CD49e antibody
see all
Database links
- Entrez Gene: 3678 Human
- Omim: 135620 Human
- SwissProt: P08648 Human
- Unigene: 505654 Human
References for Anti-Integrin alpha 5 antibody [JBS5] (ab23589)
This product has been referenced in:
- Martino MMet al. Controlling integrin specificity and stem cell differentiation in 2D and 3D environments through regulation of fibronectin domain stability. Biomaterials 30:1089-97 (2009).Read more (PubMed: 19027948) »
- Svennersten Ket al. Electrochemical modulation of epithelia formation using conducting polymers. Biomaterials 30:6257-64 (2009). ICC/IF; Dog.Read more (PubMed: 19695696) »
See all 3 publications for this product
Publishing research using ab23589? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"