Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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ab6131 at 1/200 staining human retinal epithelial cells by ICC/IF. The cells were permeabilized with 100% methanol and blocked with 1%BSA / 4% goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit IgG (H+L) was used as the secondary.
This image is courtesy of an anonymous Abreview
ab6131 staining Integrin alpha 5 in Human synovial fibroblasts by Flow Cytometry. Cells were detached by accutase, spun and fixed in 1,5 % formalin for 10 min. Gating was done on synovial fibroblasts. The sample was incubated with the primary antibody, dilution 1/500 (1% BSA in PBS), for 20 minutes at 25°C. A PE-conjugated Goat polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody. The flow cytometry plot is an overlay of the isotype control ( IgG2b, red peak) with the sample (white peak); (equal amounts of isotype IgG2b and antibody were used).
This image is courtesy of an Abreview submitted by Dr Torsten Lowin
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