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Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)
![Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)](/ps/datasheet/images/ab6131_1.jpg)
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Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)
![Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)](/ps/datasheet/images/6/ab6131/Integrin-alpha-5-Primary-antibodies-ab6131-2.jpg)
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Product Name
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Integrin alpha 5 antibody [SAM-1]
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See all Integrin alpha 5 antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [SAM-1] to Integrin alpha 5
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Immunogen
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Integrin alpha 5 positive cell preparation.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 0.5-1µg for 106 cells.
ICC/IF: 1/200 (see Abreview).
IP: Use at a concentration of 0.5 - 2 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Relevance
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Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling.
Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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SAM-1
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Isotype
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IgG2b
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS (pH 7.4) Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 2.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin alpha 5 antibody [SAM-1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Integrin alpha 5 antibody [SAM-1] images, references, products related to ab6131 and other tools.
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Integrin alpha 5 antibody [SAM-1] images:
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 Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)
ab6131 at 1/200 staining human retinal epithelial cells by ICC/IF. The cells were permeabilized with 100% methanol and blocked with 1%BSA / 4% goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit IgG (H+L) was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)
ab6131 staining Integrin alpha 5 in Human synovial fibroblasts by Flow Cytometry. Cells were detached by accutase, spun and fixed in 1,5 % formalin for 10 min. Gating was done on synovial fibroblasts. The sample was incubated with the primary antibody, dilution 1/500 (1% BSA in PBS), for 20 minutes at 25°C. A PE-conjugated Goat polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody. The flow cytometry plot is an overlay of the isotype control ( IgG2b, red peak) with the sample (white peak); (equal amounts of isotype IgG2b and antibody were used). This image is courtesy of an Abreview submitted by Dr Torsten Lowin
See Abreview
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Search PubMed (MEDLINE) for references to Integrin alpha 5
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Integrin alpha 5 antibody [SAM-1] - more information
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Customer reviews (feedback) regarding Integrin alpha 5 antibody [SAM-1] |
Customer FAQs regarding Integrin alpha 5 antibody [SAM-1] |
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Integrin alpha 5 antibody [SAM-1] - related products:
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Integrin alpha 5 antibody [SAM-1] - other tools:
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Contact Abcam with a Technical Enquiry about Integrin alpha 5 antibody [SAM-1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)](/index.html?datasheet=6131#ab6131_1.jpg)
![Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)](/index.html?datasheet=6131#Integrin-alpha-5-Primary-antibodies-ab6131-2.jpg)
