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Anti-Integrin alpha 5 antibody [SAM-1] (ab6131)

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Overview

Product name

Anti-Integrin alpha 5 antibody [SAM-1]
See all Integrin alpha 5 products (19) ...

Description

Mouse monoclonal [SAM-1] to Integrin alpha 5

Tested applications

Flow Cyt, IP, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Integrin alpha 5 positive cell preparation.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS (pH 7.4)

Concentration

Concentration information loading...

Purity

Protein G purified

Primary antibody notes

Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.

Clonality

Monoclonal

Clone number

SAM-1

Isotype

IgG2b

Research areas

Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

  • Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131) image (enlarge)

  • Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Sequence similarities

Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.

Cellular localization

Membrane.

Target information above from: UniProt accessionP08648 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD49 antigen-like family member E antibody
  • CD49e antibody
  • Fibronectin Receptor alpha antibody
  • Fibronectin receptor subunit alpha antibody
  • Fibronectin receptor, alpha subunit antibody
  • FNRA antibody
  • Integrin alpha 5 (fibronectin receptor alpha) antibody
  • Integrin alpha-5 antibody
  • Integrin alpha-5 light chain antibody
  • Integrin alpha-F antibody
  • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
  • ITA5_HUMAN antibody
  • ITGA5 antibody
  • Very Late Activation Protein 5 alpha antibody
  • Very late activation protein 5, alpha subunit antibody
  • VLA-5 antibody
  • VLA5 antibody
  • VLA5A antibody
see all

Database links

Anti-Integrin alpha 5 antibody [SAM-1] images:

  Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)

Immunocytochemistry/ Immunofluorescence - Integrin alpha 5 antibody [SAM-1] (ab6131)

ab6131 at 1/200 staining human retinal epithelial cells by ICC/IF. The cells were permeabilized with 100% methanol and blocked with 1%BSA / 4% goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit IgG (H+L) was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

  Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)

Flow Cytometry - Integrin alpha 5 antibody [SAM-1] (ab6131)

ab6131 staining Integrin alpha 5 in Human synovial fibroblasts by Flow Cytometry. Cells were detached by accutase, spun and fixed in 1,5 % formalin for 10 min.  Gating was done on synovial fibroblasts. The  sample was incubated with the primary antibody, dilution 1/500 (1% BSA in PBS), for 20 minutes at 25°C.  A PE-conjugated Goat polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody. The flow cytometry plot is an overlay of the isotype control ( IgG2b, red peak) with the sample (white peak); (equal amounts of isotype IgG2b and antibody were used).

This image is courtesy of an Abreview submitted by Dr Torsten Lowin

See Abreview

References for Anti-Integrin alpha 5 antibody [SAM-1] (ab6131)

This product has been referenced in:

  • Rowland TJet al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev 19:1231-40 (2010). ICC/IF; Human.Read more (PubMed: 19811096) »

See 1 publication for this product

Publishing research using ab6131? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"