RabMAb

Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

Overview

  • Product nameAnti-Integrin alpha 6 antibody [EPR5578]
    See all Integrin alpha 6 primary antibodies
  • Description
    Rabbit monoclonal [EPR5578] to Integrin alpha 6
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to residues in the extracellular domain of Human Integrin alpha 6.

  • Positive control
    • JAR, HT1376, and Human platelet lysates; Human kidney tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionIntegrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.
    • Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
    • Involvement in diseaseEpidermolysis bullosa letalis, with pyloric atresia
    • Sequence similaritiesBelongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Post-translational
      modifications
      Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD49 antigen-like family member F antibody
      • CD49f antibody
      • Integrin alpha-6 light chain antibody
      • Integrin alpha6B antibody
      • Integrin, alpha 6 antibody
      • ITA6_HUMAN antibody
      • ITGA6 antibody
      • ITGA6B antibody
      • VLA 6 antibody
      • VLA-6 antibody
      see all

    Anti-Integrin alpha 6 antibody [EPR5578] images

    • All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

      Lane 1 : JAR cell lysates
      Lane 2 : HT1376 cell lysates
      Lane 3 : Human platelet lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 126 kDa

      Secondary antibody - anti-rabbit HRP (ab6721)

    • ab124924, at 1/100 dilution, staining Integrin alpha 6 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

    This product has been referenced in:
    • Suprynowicz FA  et al. Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells. Proc Natl Acad Sci U S A 109:20035-40 (2012). WB ; Human . Read more (PubMed: 23169653) »
    • Kariya Y & Gu J N-glycosylation of ß4 integrin controls the adhesion and motility of keratinocytes. PLoS One 6:e27084 (2011). WB . Read more (PubMed: 22073258) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Sample Mouse Cell (mouse embryonic stem cells)
    Specification mouse embryonic stem cells
    Permeabilization Yes - 0.1% triton x
    Fixative methanol:acetone 1:1
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2014

    Application Western blot
    Loading amount 1e+006 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Specification mouse embryonic stem cell
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"