Recombinant Anti-CD103 antibody [EPR4166] (ab108503)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4166] to CD103
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD103 antibody [EPR4166]
See all CD103 primary antibodies -
Description
Rabbit monoclonal [EPR4166] to CD103 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HuT-78, Jurkat, 431, HeLa and K562 cell lysates.
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General notes
Clone EPR4166 is different from clone EPR4166(2).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4166 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa). |
Target
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Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. -
Tissue specificity
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3682 Human
- Omim: 604682 Human
- SwissProt: P38570 Human
- Unigene: 513867 Human
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Alternative names
- A530055J10 antibody
- alpha-E1 antibody
- alpha-M290 antibody
see all
Images
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All lanes : Anti-CD103 antibody [EPR4166] (ab108503) at 1/1000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab108503 has been referenced in 3 publications.
- Ishibashi H et al. Endoscopic and clinicopathological characteristics of gastrointestinal adult T-cell leukemia/lymphoma. J Gastrointest Oncol 10:723-733 (2019). PubMed: 31392053
- Weisberg SP et al. Tissue-Resident Memory T Cells Mediate Immune Homeostasis in the Human Pancreas through the PD-1/PD-L1 Pathway. Cell Rep 29:3916-3932.e5 (2019). PubMed: 31851923
- Ishibashi H et al. Multiple lesions of gastrointestinal tract invasion by monomorphic epitheliotropic intestinal T-cell lymphoma, accompanied by duodenal and intestinal enteropathy-like lesions and microscopic lymphocytic proctocolitis: a case series. Diagn Pathol 11:66 (2016). IHC-P ; Human . PubMed: 27457239