Anti-Integrin beta 1 antibody [4B7R] (ab3167)

Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [4B7R] (ab3167)

Image courtesy of Human Protein Atlas

ab3167 staining Integrin beta 1 in Human skin, showing a distinct and strong staining pattern at the basal cells of the squamous epithelium that contrasts with the reticular layer. Paraffin embedded human skin tissue was incubated with ab3167 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab3167 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

Western blot - Integrin beta 1 antibody [4B7R] (ab3167)

Anti-Integrin beta 1 antibody [4B7R] (ab3167) at 5 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 88 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

It is probable that this protein runs higher than the predicted MW as it likely to be glycosylated.

Flow Cytometry - Integrin beta 1 antibody [4B7R] (ab3167)

Overlay histogram showing MCF-7 cells stained with ab3167 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3167, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/250 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.