![Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [4B7R] (ab3167) image](#ab3167_1_skinII.jpg)
![Western blot - Integrin beta 1 antibody [4B7R] (ab3167) image](#Integrin-beta-1-Primary-antibodies-ab3167-2.jpg)
![Flow Cytometry - Integrin beta 1 antibody [4B7R] (ab3167) image](#Integrin-beta-1-Primary-antibodies-ab3167-3.jpg)
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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-Integrin beta 1 antibody [4B7R]
See all Integrin beta 1 products (40) ...
Description
Mouse monoclonal [4B7R] to Integrin beta 1
Tested applications
WB, Flow Cyt, IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Human melanoma V+B2 cell line.
Positive control
Tonsil.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
4B7R
Isotype
IgG1
Light chain type
kappa
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
Flow Cyt: Use 1µg for 106 cells. ((paraformaldehyde or methanol fixed cells))
IF: Use at an assay dependent dilution.
IHC-P: Use a concentration of 10 - 20 µg/ml.Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Target
Function
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis.
Tissue specificity
Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
Post-translational
modifications
The cysteine residues are involved in intrachain disulfide bonds.
Cellular localization
Cell membrane. Melanosome. Cleavage furrow. Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in afew intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells.
Target information above from: UniProt accessionP05556
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- beta 1 subunit antibody
- CD 29 antibody
- CD29 antibody
see all
Database links
- Entrez Gene: 3688 Human
- Omim: 135630 Human
- SwissProt: P05556 Human
- Unigene: 643813 Human
Anti-Integrin beta 1 antibody [4B7R] images:
Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [4B7R] (ab3167)
![Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [4B7R] (ab3167)](/ps/datasheet/Images/3/ab3167/ab3167_1_skinII.jpg)
Image courtesy of Human Protein Atlas
ab3167 staining Integrin beta 1 in Human skin, showing a distinct and strong staining pattern at the basal cells of the squamous epithelium that contrasts with the reticular layer. Paraffin embedded human skin tissue was incubated with ab3167 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab3167 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Western blot - Integrin beta 1 antibody [4B7R] (ab3167)
![Western blot - Integrin beta 1 antibody [4B7R] (ab3167)](/ps/datasheet/images/3/ab3167/Integrin-beta-1-Primary-antibodies-ab3167-2.jpg)
Anti-Integrin beta 1 antibody [4B7R] (ab3167) at 5 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 88 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,40 kDa,50 kDa. We are unsure as to the identity of these extra bands.
It is probable that this protein runs higher than the predicted MW as it likely to be glycosylated.
Flow Cytometry - Integrin beta 1 antibody [4B7R] (ab3167)
![Flow Cytometry - Integrin beta 1 antibody [4B7R] (ab3167)](/ps/datasheet/images/3/ab3167/Integrin-beta-1-Primary-antibodies-ab3167-3.jpg)
Overlay histogram showing MCF-7 cells stained with ab3167 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3167, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-Integrin beta 1 antibody [4B7R] (ab3167)
This product has been referenced in:
- Lai-Cheong JEet al. Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activation. Am J Pathol 175:1431-41 (2009). IF; Human.Read more (PubMed: 19762710) »
- Di Marco GSet al. Increased inorganic phosphate induces human endothelial cell apoptosis in vitro. Am J Physiol Renal Physiol : (2008).Read more (PubMed: 18385273) »
See all 5 publications for this product
Publishing research using ab3167? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"