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Western blot - Integrin beta 1 antibody [EP1041Y] (ab52971)
![Western blot - Integrin beta 1 antibody [EP1041Y] (ab52971)](/ps/datasheet/images/ab52971_WB_1.jpg)
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Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] (ab52971)
![Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] (ab52971)](/ps/datasheet/images/ab52971_IHC_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)](/ps/datasheet/images/ab52971_1.jpg)
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Product Name
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Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end
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See all Integrin beta 1 antibodies (35)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP1041Y] to Integrin beta 1 - Carboxyterminal end
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Immunogen
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A synthetic peptide corresponding to the residue near the C-terminal of human Integrin beta 1 was used as immunogen
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/70.
IHC-P: 1/250 - 1/500. Antigen retrieval step and use of an HRP/AP polymerized secondary antibody recommended.
WB: 1/500. Detects a band of approximately 140-150 kDa (predicted molecular weight: 88 kDa).
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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U937 cell membrane lysate
Human breast carcinoma
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Cellular localization
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Type 1 membrane protein
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Relevance
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Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP1041Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, Tissue culture supernatant, 0.18M Sodium chloride, 50mM Tris glycine, 25mM Citric acid, 0.25mM EDTA, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end images, references, products related to ab52971 and other tools.
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Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end images:
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 Western blot - Integrin beta 1 antibody [EP1041Y] (ab52971)
Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971) at 1/500 dilution + U937 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size : 88 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] (ab52971)
ab52971, at a 1/250 dilution, staining Human Integrin beta 1 in breast carcinoma using Immunohistochemistry, Paraffin Embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)
ab52971 staining human breast cancer metastasis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with a commercial blocking reagent and incubation with the antibody (diluted 1/100) for 18 hours at 4°C. A HRP-conjugated goat anti-rabbit was used as the secondary antibody. This image shows a cancer metastasis at 40x with beta1 staining (in red) in both blood vessels and tumour cells. Blue is Hoechst for nuclei. The antibody detection was enhanced using a commercial Cy3 tyramide signal amplification kit. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)
![Immunocytochemistry/ Immunofluorescence - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)](\ps\datasheet\images\52\ab52971\Integrin-beta-1-Primary-antibodies-ab52971-3.jpg)
ICC/IF image of ab52971 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52971, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)
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This product has been used in:
Chen TT et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
If you publish research using ab52971 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Integrin beta 1
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Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end - more information
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Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end - related products:
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Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end - other tools:
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Contact Abcam with a Technical Enquiry about Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Integrin beta 1 antibody [EP1041Y] (ab52971)](/index.html?datasheet=52971#ab52971_WB_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] (ab52971)](/index.html?datasheet=52971#ab52971_IHC_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)](/index.html?datasheet=52971#ab52971_1.jpg)
