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Anti-Integrin beta 1 antibody [P4G11]
See all Integrin beta 1 products (40) ...
Mouse monoclonal [P4G11] to Integrin beta 1
ab78502 reacts with calcium-dependent epitopes on the integrin beta 1 subunit. When bound at intergrin beta 1, ab78502 activates intergrin beta 1 dependent binding to extra cellular matrix substrates.
IP, IHC-Fr, ICC, Flow Cyt, IHC-P, ELISA, IHC-FoFr, Functional Studiesmore details
Reacts with
Rabbit, Human, Monkey
Human IL-1beta activated endothelial cells
Tonsil and human skin tissue.
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6
Concentration information loading...
Protein A purified
Monoclonal
P4G11
IgG1
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Our Abpromise guarantee covers the use of ab78502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use 2-5µg for 106 cells in the presence of Ca2+.
FuncS: Stimulation of cells: 2-10 µg/ml for 106 cells. Binding buffer must contain 1mM Ca2+. Stimulates ECM Adhesion.
ICC: Use at a concentration of 2 µg/ml (for use on numerous cell lines as long as Ca2+ is present).
IHC-Fr: Use at an assay dependent dilution. Use acetone fixed tissue. Ca2+ required for binding. Ca2+ must be used in blocking, washing, and antibody incubation buffers.
IHC-P: 1/500. Use 4% PFA fixed tissue. Use citrate HEIR treatment. Ca2+ required for binding. Ca2+ must be used in blocking, washing, and antibody incubation buffers.
IHC-FoFr: Use at an assay dependent dilution. Use mild paraformaldehyde (2-2.5% PFA, short time) fixed tissue. Ca2+ required for binding. Ca2+ must be used in blocking, washing, and antibody incubation buffers.
IP: Use at a concentration of 5 µg/400µl lysate (0.5% triton PBS plus Ca2+).
Is unsuitable traditional formalin-fixed IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis.
Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).
Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
The cysteine residues are involved in intrachain disulfide bonds.
Cell membrane. Melanosome. Cleavage furrow. Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in afew intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells.
Target information above from: UniProt accessionP05556
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
ab78502 has not yet been referenced specifically in any publications.
Publishing research using ab78502? Please let us know so that we can cite the reference in this datasheet
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