Feedback Form

Product Name 

Integrin beta 1 antibody [P4G11]

 

See all Integrin beta 1 antibodies (34)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [P4G11] to Integrin beta 1

Immunogen 

Human IL-1beta activated endothelial cells

Reacts with 
(species key)

Hu, Mk, Rb

Specificity 

ab78502 reacts with calcium-dependent epitopes on the integrin beta 1 subunit. When bound at intergrin beta 1, ab78502 activates intergrin beta 1 dependent binding to extra cellular matrix substrates.

Tested applications 
(see key)

ELISA, Flow Cyt, FuncS, ICC, IHC-FoFr, IHC-Fr, IHC-P, IP


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use 2-5µg for 106 cells in the presence of Ca2+.
FuncS: Stimulation of cells: 2-10 µg/ml for 106 cells. Binding buffer must contain 1mM Ca2+. Stimulates ECM Adhesion.
ICC: Use at a concentration of 2 µg/ml (for use on numerous cell lines as long as Ca2+ is present).
IHC-Fr: Use at an assay dependent dilution. Use acetone fixed tissue. Ca2+ required for binding. Ca2+ must be used in blocking, washing, and antibody incubation buffers.
IHC-P: 1/500. Use 4% PFA fixed tissue. Use citrate HEIR treatment. Ca2+ required for binding. Ca2+ must be used in blocking, washing, and antibody incubation buffers.
IHC-FoFr: Use at an assay dependent dilution. Use mild paraformaldehyde (2-2.5% PFA, short time) fixed tissue. Ca2+ required for binding. Ca2+ must be used in blocking, washing, and antibody incubation buffers.
IP: Use at a concentration of 5 µg/400µl lysate (0.5% triton PBS plus Ca2+).

Is unsuitable traditional formalin-fixed IHC-P.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil and human skin tissue.

Cellular localization 

Type 1 membrane protein

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

 

Alternative names Integrin beta 1 antibody [P4G11] (ab78502)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3688    (Human)

GeneCard

    

GC10M032936    (Human)

Omim

    

135630    (Human)

SwissProt

    

P05556    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

P4G11

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin beta 1 antibody [P4G11] is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to Integrin beta 1

 

Integrin beta 1 antibody [P4G11] - more information

Customer reviews (feedback) regarding Integrin beta 1 antibody [P4G11]

Customer FAQs regarding Integrin beta 1 antibody [P4G11]

Protocols for Integrin beta 1 antibody [P4G11]

Price and availability of products related to Integrin beta 1 antibody [P4G11]

 

Integrin beta 1 antibody [P4G11] - related products:

Products for Immunocytochemistry

Subcellular Markers

 

Compatible Secondaries for Integrin beta 1 antibody [P4G11]

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other Integrin beta 1 Antibodies

See all Integrin beta 1 antibodies (34)...

 

Integrin beta 1 antibody [P4G11] - other tools:

Contact Abcam with a Technical Enquiry about Integrin beta 1 antibody [P4G11]

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.