Integrin beta 1 antibody [P4G11] (ab78502)

Product Name 

Integrin beta 1 antibody [P4G11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [P4G11] to Integrin beta 1

Immunogen 

Human IL-1beta activated endothelial cells

Reacts with 
(species key)

Hu, Mk, Rb

Specificity 

ab78502 reacts with calcium-dependent epitopes on the integrin beta 1 subunit. When bound at intergrin beta 1, ab78502 activates intergrin beta 1 dependent binding to extra cellular matrix substrates.

Tested applications 
(see key)

ELISA, Flow Cyt, FuncS, ICC, IHC-FoFr, IHC-Fr, IHC-P, IP

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use 2-5µg for 10⁶ cells in the presence of Ca²⁺.
FuncS: Stimulation of cells: 2-10 µg/ml for 10⁶ cells. Binding buffer must contain 1mM Ca²⁺. Stimulates ECM Adhesion.
ICC: Use at a concentration of 2 µg/ml (for use on numerous cell lines as long as Ca²⁺ is present).
IHC-Fr: Use at an assay dependent dilution. Use acetone fixed tissue. Ca²⁺ required for binding. Ca²⁺ must be used in blocking, washing, and antibody incubation buffers.
IHC-P: 1/500. Use 4% PFA fixed tissue. Use citrate HEIR treatment. Ca²⁺ required for binding. Ca²⁺ must be used in blocking, washing, and antibody incubation buffers.
IHC-FoFr: Use at an assay dependent dilution. Use mild paraformaldehyde (2-2.5% PFA, short time) fixed tissue. Ca²⁺ required for binding. Ca²⁺ must be used in blocking, washing, and antibody incubation buffers.
IP: Use at a concentration of 5 µg/400µl lysate (0.5% triton PBS plus Ca²⁺).

Is unsuitable traditional formalin-fixed IHC-P.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil and human skin tissue.

Cellular localization 

Type 1 membrane protein

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

P4G11

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

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