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Anti-Integrin beta 1 antibody [k2D5] (ab72843) at 1/500 dilution + U937 cell lysate at 40 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
developed using the ECL technique
Predicted band size : 88 kDa
Observed band size : 131 kDa (why is the actual band size different from the predicted?)
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