Integrin beta 3 antibody [CRC54] (ab34409)

Product Name 

Integrin beta 3 antibody [CRC54]

Product type 

Primary antibodies

Description 

Mouse monoclonal [CRC54] to Integrin beta 3

Immunogen 

Purified human gpIIIa/VNR beta-chain glycoprotein.

Reacts with 
(species key)

Hu

Specificity 

The antibody reacts with platelets and megakaryocytes. The antibody reacts preferentially with the activated/occupied form of platelet GPIIb-IIIa (ligand- induced biding site or LIBS epitope) and dissociated form of GPIIIa. This antibody does not react with platelets of patients with Glanzmann thrombastenia.

Epitope 

The epitope is located within the N-terminal region of GPIIIa (first 100 amino acids).

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 87 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein

Research areas 

Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Integrins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins

Relevance 

Human Integrin beta 3 (CD61) antigen is a 90-110 kDa glycoprotein polypeptide which is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells. The platelet glycoprotein is the integrin beta 3 chain of the vitronectin receptor and GPIIb/IIIa complex.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

CRC54

Isotype 

IgG1

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Ammonium sulfate + DEAE-cellulose, gradient elution

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for Integrin beta 3 antibody [CRC54] (ab34409)

  This product has been used in: (two most recent references)

Alghisi GC et al. The integrin antagonist cilengitide activates alphaVbeta3, disrupts VE-cadherin localization at cell junctions and enhances permeability in endothelial cells. PLoS One 4:e4449 (2009). ICC/IF; Human. PubMed: 19212436

Reynolds AR et al. Stimulation of tumor growth and angiogenesis by low concentrations of RGD-mimetic integrin inhibitors. Nat Med 15:392-400 (2009). Flow Cyt; Human. PubMed: 19305413

See all 3 publication references for this product.


If you publish research using ab34409 please let us know so that we can cite the reference on this datasheet.

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