Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EP2417Y] (ab75872)

ab75872 staining mouse brain sections by IHC-P. The tissue was fixed with paraformaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Permeabilization was performed with 0.1% Tween and blocking of the sample was done with PBS/0.1% Tween/5% goat serum for 1 hour at 25°C, followed by staining with ab75872 at 1/250 in PBS/0.1% Tween/5% goat serum/azide for 12h at 4°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Upper panel shows a section from a WT animal and bottom panel shows a section from an animal with full knockout to the Itgb3 gene. Integrin beta3 staining was observed in NeuN positive cells.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 3 antibody [EP2417Y] (ab75872)

ab75872 at 1/100 dilution staining Integrin beta 3 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

Western blot - Integrin beta 3 antibody [EP2417Y] (ab75872)

Anti-Integrin beta 3 antibody [EP2417Y] (ab75872) at 1/2000 dilution + human spleen lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size : 87 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)

Immunohistochemistry (Frozen sections) - Integrin beta 3 antibody [EP2417Y] (ab75872)

ab75872 staining Integrin beta 3 in Human pancreatic PanIN lesion tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with 5% Serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in PBS and incubated with the sample for 8 hours at 4ºC. An Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.

This image is courtesy of an anonymous Abreview.

Immunohistochemistry (Frozen sections) - Integrin beta 3 antibody [EP2417Y] (ab75872)

ab75872 staining Integrin beta 3 in Mouse pancreatic tumor tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with 5% Serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in PBS and incubated with the sample for 8 hours at 4ºC. An Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.

This image is courtesy of an anonymous Abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EP2417Y] (ab75872)

Mouse vessel stained using IHC-P. Recommended dilution for Mouse tissue is 1:100-250.