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Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)

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Overview

Product name

Anti-Integrin beta 3 (phospho Y773) antibody
See all Integrin beta 3 products (31) ...

Description

Rabbit polyclonal to Integrin beta 3 (phospho Y773)

Specificity

ab38460 recognises endogenous levels of Integrin beta 3 only when phosphorylated at tyrosine 773.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic phosphopeptide derived from human Integrin beta 3 around the phosphorylation site of tyrosine 773 (PLYpKE)

Positive control

Human breast carcinoma tissue, HL-60 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/10,000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 87 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Tissue specificity

Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Involvement in disease

Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.

Sequence similarities

Belongs to the integrin beta chain family.
Contains 1 VWFA domain.

Post-translational
modifications

Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.

Cellular localization

Membrane.

Target information above from: UniProt accessionP05106 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BDPLT2 antibody
  • CD 61 antibody
  • CD61 antibody
  • CD61 antigen antibody
  • GP3A antibody
  • GPIIIa antibody
  • GT antibody
  • HPA 1 antibody
  • HPA 4 antibody
  • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
  • Integrin beta chain beta 3 antibody
  • Integrin beta-3 antibody
  • ITB3_HUMAN antibody
  • ITG B3 antibody
  • ITGB 3 antibody
  • ITGB3 antibody
  • NAIT antibody
  • Platelet fibrinogen receptor beta subunit antibody
  • Platelet glycoprotein IIIa antibody
  • platelet glycoprotein IIIa precursor antibody
  • Platelet membrane glycoprotein IIIa antibody
  • PTP antibody
see all

Anti-Integrin beta 3 (phospho Y773) antibody images:

  Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)

Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)

All lanes : Anti-Integrin beta 3 (phospho Y773) antibody (ab38460) at 1/500 dilution

Lane 1 : Untreated extracts from HL-60 cells
Lane 2 : Hydrogen peroxide treated extracts from HL-60 cells

Lysates/proteins at 30 µg per lane.


Predicted band size : 87 kDa
Observed band size : >118 kDa (why is the actual band size different from the predicted?)


Lanes can be loaded with 5-30µg of total protein.

  Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)

Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)

Immunohistochemical analysis of Integrin beta 3 (phospho Y773) expression in paraffin embedded human breast carcinoma tissue sections using 1/50 ab38460. Left: untreated sample. Right: Sample pretreated with phosphopeptide (negative control).

References for Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)

ab38460 has not yet been referenced specifically in any publications.

Publishing research using ab38460? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"