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Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)
Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)
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Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)
Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)
(enlarge)


Product Name 

Integrin beta 3 (phospho Y773) antibody

 

See all Integrin beta 3 antibodies (29)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Integrin beta 3 (phospho Y773)

Immunogen 

Synthetic phosphopeptide derived from human Integrin beta 3 around the phosphorylation site of tyrosine 773 (PLYpKE)

Reacts with 
(species key)

Hu, Ms

Specificity 

ab38460 recognises endogenous levels of Integrin beta 3 only when phosphorylated at tyrosine 773.

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/10,000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 87 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast carcinoma tissue, HL-60 cells.

Cellular localization 

Type I membrane protein

Research areas 

Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Integrins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins

Relevance 

Human Integrin beta 3 (CD61) antigen is a 90-110 kDa glycoprotein polypeptide which is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells. The platelet glycoprotein is the integrin beta 3 chain of the vitronectin receptor and GPIIb/IIIa complex.

 

Alternative names Integrin beta 3 (phospho Y773) antibody (ab38460)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3690    (Human)
16416    (Mouse)

Omim

    

173470    (Human)

SwissProt

    

P05106    (Human)
O54890    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin beta 3 (phospho Y773) antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Integrin beta 3 (phospho Y773) antibody images, references, products related to ab38460 and other tools.

 

Integrin beta 3 (phospho Y773) antibody images:

Product image  Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)

Western blot - Integrin beta 3 (phospho Y773) antibody (ab38460)

All lanes : Integrin beta 3 (phospho Y773) antibody (ab38460) at 1/500 dilution

Lane 1 : Untreated extracts from HL-60 cells
Lane 2 : Hydrogen peroxide treated extracts from HL-60 cells

Lysates/proteins at 30 µg per lane.


Predicted band size : 87 kDa
Observed band size : >118 kDa (why is the actual band size different from the predicted?)


Lanes can be loaded with 5-30µg of total protein.

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)

Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 3 (phospho Y773) antibody (ab38460)

Immunohistochemical analysis of Integrin beta 3 (phospho Y773) expression in paraffin embedded human breast carcinoma tissue sections using 1/50 ab38460. Left: untreated sample. Right: Sample pretreated with phosphopeptide (negative control).

 
 

Search PubMed (MEDLINE) for references to Integrin beta 3

 

Integrin beta 3 (phospho Y773) antibody - more information

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Price and availability of products related to Integrin beta 3 (phospho Y773) antibody

 

Integrin beta 3 (phospho Y773) antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Corresponding non-phospho antibody for Integrin beta 3 (phospho Y773) antibody

ab47387

  

Rabbit polyclonal to Integrin beta 3

ab47584

  

Rabbit polyclonal to Integrin beta 3

 

Positive Controls for Integrin beta 3 (phospho Y773) antibody

ab7914

  

HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate

 

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Other Integrin beta 3 Antibodies

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