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Product name
Anti-Integrin beta 3 (phospho Y785) antibody
See all Integrin beta 3 products (30) ...
Description
Rabbit polyclonal to Integrin beta 3 (phospho Y785)
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the c-terminus of the Integrin beta 3 receptor (based on Swiss Protein database, accession number P05106). The sequence is conserved in human, mouse and rat.
Positive control
K562 cells transfected with human Integrin alpha v and wild-type (WT) or mutant (Y785F) human Integrin beta 3, and analysed by immunoprecipitation with 1A2 (human Integrin beta 3) mAb.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Integrin beta 3 receptor protein. The final product is generated by affinity chromatography using an Integrin beta 3 receptor-derived peptide that is phosphorylated at tyrosine 785 (tyrosine 759 in the chicken sequence for Integrin beta 3).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Integrins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 130 kDa.
Target
Function
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Tissue specificity
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Involvement in disease
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
Post-translational
modifications
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BDPLT2 antibody
- CD 61 antibody
- CD61 antibody
see all
Database links
- Entrez Gene: 374209 Chicken
- Entrez Gene: 3690 Human
- Entrez Gene: 16416 Mouse
- Entrez Gene: 29302 Rat
- Omim: 173470 Human
- SwissProt: P05106 Human
- SwissProt: O54890 Mouse
- Unigene: 218040 Human
see all
Anti-Integrin beta 3 (phospho Y785) antibody images:
Western Blot: K562 cells transfected with human Integrin alpha v and wild type (WT) or mutant (Y785F) human Integrin beta 3 were analyzed by immunoprecipitating the Integrin beta 3 protein with 1A2 (human Integrin beta 3) mAb, and resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to PVDF and, after blocking with a 5% BSA TBST buffer overnight at 4oC, incubated with 0.50 µg/mL Integrin beta 3 [pY785] antibody with samples corresponding to: non-phosphorylated Integrin beta 3 (1), phosphorylated WT Integrin beta 3 (2), or phosphorylated Y785F mutant Integrin beta 3 (3). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show detection of Integrin beta 3 receptor phosphorylation with the wild-type but not the mutant recombinant protein, thereby demonstrating the specificity of the Integrin beta 3 [pY785] antibody.
References for Anti-Integrin beta 3 (phospho Y785) antibody (ab5191)
ab5191 has not yet been referenced specifically in any publications.
Publishing research using ab5191? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"