You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Integrin beta 4 antibody [346-11A] (ab25254)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Integrin beta 4 antibody [346-11A]
See all Integrin beta 4 products (16) ...

Description

Rat monoclonal [346-11A] to Integrin beta 4

Specificity

ab25254 recognises Integrin ß4 chain (CD104), Mr 200 kDa.

Tested applications

ICC/IF, IHC-Fr, IP, WBmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

100mM borate buffered saline, pH 8.0.

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

346-11A

Isotype

IgG2a

Light chain type

kappa

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta

  • Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.

Tissue specificity

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

Involvement in disease

Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

Sequence similarities

Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.

Domain

The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

Cellular localization

Membrane.

Target information above from: UniProt accessionP16144 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 104 antibody
  • CD104 antibody
  • CD104 antigen antibody
  • GP150 antibody
  • Integrin beta-4 antibody
  • ITB4_HUMAN antibody
  • ITG B4 antibody
  • ITGB 4 antibody
  • ITGB4 antibody
see all

Database links

Anti-Integrin beta 4 antibody [346-11A] images:

  Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)

Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)

This image shows a acetone fixed frozen section of BALB/c mouse intestine. The basal layer stains intensely (arrow).

  Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)

Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)

ab25254 staining mouse small intestine tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C.  A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Integrin beta 4 antibody [346-11A] (ab25254)

This product has been referenced in:

  • Oberle Cet al. Lysosomal membrane permeabilization and cathepsin release is a Bax/Bak-dependent, amplifying event of apoptosis in fibroblasts and monocytes. Cell Death Differ 17:1167-78 (2010). ICC/IF; Mouse.Read more (PubMed: 20094062) »
  • Sachs Net al. Kidney failure in mice lacking the tetraspanin CD151. J Cell Biol 175:33-9 (2006).Read more (PubMed: 17015618) »

See all 3 publications for this product

Publishing research using ab25254? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"