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Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)
![Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)](/ps/datasheet/images/Ab25254b.png)
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Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)
![Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)](/ps/datasheet/images/ab25254_1.jpg)
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Product Name
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Integrin beta 4 antibody [346-11A]
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See all Integrin beta 4 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [346-11A] to Integrin beta 4
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Immunogen
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Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.
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Reacts with
(species key)
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Ms
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Specificity
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ab25254 recognises Integrin ß4 chain (CD104), Mr 200 kDa.
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Tested applications
(see key)
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IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: Use at a concentration of 1-5 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 202 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; single pass type I membrane protein
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
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Relevance
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Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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346-11A
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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100mM borate buffered saline, pH 8.0.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin beta 4 antibody [346-11A] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Integrin beta 4 antibody [346-11A] images, references, products related to ab25254 and other tools.
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Integrin beta 4 antibody [346-11A] images:
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 Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)
This image shows a acetone fixed frozen section of BALB/c mouse intestine. The basal layer stains intensely (arrow).
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Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)
ab25254 staining mouse small intestine tissue sections by IHC-Fr. Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C. A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for Integrin beta 4 antibody [346-11A] (ab25254)
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This product has been used in:
Sachs N et al. Kidney failure in mice lacking the tetraspanin CD151. J Cell Biol 175:33-9 (2006). PubMed: 17015618
If you publish research using ab25254 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Integrin beta 4
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Integrin beta 4 antibody [346-11A] - more information
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Customer reviews (feedback) regarding Integrin beta 4 antibody [346-11A] |
Customer FAQs regarding Integrin beta 4 antibody [346-11A] |
Protocols for Integrin beta 4 antibody [346-11A] |
Price and availability of products related to Integrin beta 4 antibody [346-11A] |
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Integrin beta 4 antibody [346-11A] - related products:
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Integrin beta 4 antibody [346-11A] - other tools:
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Contact Abcam with a Technical Enquiry about Integrin beta 4 antibody [346-11A] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)](/index.html?datasheet=25254#Ab25254b.png)
![Immunohistochemistry (Frozen sections) - Integrin beta 4 antibody [346-11A] (ab25254)](/index.html?datasheet=25254#ab25254_1.jpg)
