You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Integrin beta 4 antibody [M126] (ab29042)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Integrin beta 4 antibody [M126]
See all Integrin beta 4 products (16) ...

Description

Mouse monoclonal [M126] to Integrin beta 4

Specificity

ab29042 recognises all three isoforms of integrin beta 4 Beta 4A, beta 4B and beta 4C.

Tested applications

ELISA, WB, ICC, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Dog, Human

Immunogen

recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.

Positive control

A431 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

M126

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta

  • Western blot - Integrin beta 4 antibody [M126] (ab29042)Western blot - Integrin beta 4 antibody [M126] (ab29042) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab29042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/2000

    ELISA: 1/2000

  • ShowHide1 Image

    WB

     WB: 1/1000Predicted mole...Read more →

    WB: 1/1000Predicted molecular weight: 202 kDa.(In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. )

  • ICC

     ICC: 1/250

    ICC: 1/250

  • ShowHide

    ICC/IF

    Three stars (1 Abreview) ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent concentration. (PubMed: 21042878)

Target

Function

Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.

Tissue specificity

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

Involvement in disease

Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

Sequence similarities

Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.

Domain

The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

Cellular localization

Membrane.

Target information above from: UniProt accessionP16144 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 104 antibody
  • CD104 antibody
  • CD104 antigen antibody
  • GP150 antibody
  • Integrin beta-4 antibody
  • ITB4_HUMAN antibody
  • ITG B4 antibody
  • ITGB 4 antibody
  • ITGB4 antibody
see all

Database links

see all

Anti-Integrin beta 4 antibody [M126] images:

  Western blot - Integrin beta 4 antibody [M126] (ab29042)

Western blot - Integrin beta 4 antibody [M126] (ab29042)

All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)

Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min


Predicted band size : 202 kDa

References for Anti-Integrin beta 4 antibody [M126] (ab29042)

This product has been referenced in:

  • Wei Wet al. Modulation of TGF-ß-inducible hypermotility by EGF and other factors in human prostate epithelial cells and keratinocytes. In Vitro Cell Dev Biol Anim 46:841-55 (2010).Read more (PubMed: 21042878) »
  • Wei Wet al. Modulation of TGF-ß-inducible hypermotility by EGF and other factors in human prostate epithelial cells and keratinocytes. In Vitro Cell Dev Biol Anim 46:841-55 (2010). ICC/IF; Human.Read more (PubMed: 21042878) »

See all 3 publications for this product

Publishing research using ab29042? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"