Integrin beta 4 antibody [M126] (ab29042)

Product Name 

Integrin beta 4 antibody [M126]

Product type 

Primary antibodies

Description 

Mouse monoclonal [M126] to Integrin beta 4

Immunogen 

recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.

Reacts with 
(species key)

Hu, Ms, Rat, Dog

Specificity 

ab29042 recognises all three isoforms of integrin beta 4 Beta 4A, beta 4B and beta 4C.

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/2000.
ICC: 1/250.
WB: 1/1000 in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 202 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 cell lysate.

Cellular localization 

Cell Membrane; single pass type I membrane protein

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta

Relevance 

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

M126

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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Integrin beta 4 antibody [M126] images:

References for Integrin beta 4 antibody [M126] (ab29042)

  This product has been used in:

Doehn U et al. RSK is a principal effector of the RAS-ERK pathway for eliciting a coordinate promotile/invasive gene program and phenotype in epithelial cells. Mol Cell 35:511-22 (2009). WB; Human, Dog. PubMed: 19716794

If you publish research using ab29042 please let us know so that we can cite the reference on this datasheet.

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