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Western blot - Integrin beta 4 (phospho Y1494) antibody (ab29043)
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Product Name
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Integrin beta 4 (phospho Y1494) antibody
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See all Integrin beta 4 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Integrin beta 4 (phospho Y1494)
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Immunogen
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Synthetic phospho peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human integrin beta 4.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab29043 recognises all three isoforms of Integrin beta 4 phosphorilated at tyrosine 1494 (beta 4A, beta 4B and beta 4C).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/4000.
WB: 1/2000 Block the membrane in 5% BSA (fraction V). Predicted molecular weight: 202 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 cells treated with pervanadate.
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Cellular localization
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Cell Membrane; single pass type I membrane protein
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
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Relevance
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Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab29043 was cross adsorbed to phospho tyrosine coupled to agarose prior to immunogen affinity purification (without carrier protein).
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin beta 4 (phospho Y1494) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Integrin beta 4 (phospho Y1494) antibody images, references, products related to ab29043 and other tools.
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Integrin beta 4 (phospho Y1494) antibody images:
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 Western blot - Integrin beta 4 (phospho Y1494) antibody (ab29043)
All lanes : Integrin beta 4 (phospho Y1494) antibody (ab29043)
Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with absence of peptide
Lane 3 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1494) peptide
Lane 4 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1526) peptide
Predicted band size : 202 kDa
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Search PubMed (MEDLINE) for references to Integrin beta 4
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Integrin beta 4 (phospho Y1494) antibody - more information
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