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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Overview
Product name
Anti-Integrin beta 4 (phospho Y1526) antibody
See all Integrin beta 4 products (16) ...
Description
Rabbit polyclonal to Integrin beta 4 (phospho Y1526)
Specificity
The immunogen sequence used is found in all three integrin beta4 isoforms and is highly conserved in rat and mouse integrin beta4.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues around tyrosine 1526 of human integrin-beta4.
Positive control
A431 and src-transformed Hct116 cells treated with pervanadate.
Properties
Form
Liquid
Storage instructions
Store at -20°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to dephosphorylated integrin beta4 (Tyr-1526) peptide before affinity purification using phospho-integrin beta-4(Tyr-1526) peptide (without carrier).
Primary antibody notes
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
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Western blot - Integrin beta 4 (phospho Y1526) antibody (ab29044)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab29044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/2000ELISA: 1/2000
WB: 1/1000Detects a band of approximately 202 kDa.
Target
Function
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in disease
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.
Domain
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16144
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
Database links
- Entrez Gene: 3691 Human
- Entrez Gene: 192897 Mouse
- Entrez Gene: 25724 Rat
- Omim: 147557 Human
- SwissProt: P16144 Human
- SwissProt: A2A863 Mouse
- SwissProt: A2A864 Mouse
- SwissProt: Q64632 Rat
see all
Anti-Integrin beta 4 (phospho Y1526) antibody images:
Western blot - Integrin beta 4 (phospho Y1526) antibody (ab29044)
Western blot analysis of A431 (25 ug/lane) serum starved overnight (lane 1) and treated with pervanadate (1 mM) for 30 min (lanes 2-4). The blot was probed with rabbit polyclonal anti-phospho-integrin-beta4 (Tyr-1526). In the pervanadate-treated lanes, the polyclonal antibodies were used in the presence of no peptide (lane 2), phospho-integrin-beta4 (Tyr-1494) peptide (lane 3), or phospho-integrin-beta4 (Tyr-1526) peptide (lane 4).
References for Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)
This product has been referenced in:
- Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB; Human.Read more (PubMed: 18978815) »
See 1 publication for this product
Publishing research using ab29044? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"