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Anti-Integrin beta 4 (phospho Y1526) antibody
See all Integrin beta 4 products (16) ...
Rabbit polyclonal to Integrin beta 4 (phospho Y1526)
The immunogen sequence used is found in all three integrin beta4 isoforms and is highly conserved in rat and mouse integrin beta4.
ELISA, WBmore details
Reacts with
Human
Predicted to work with
Mouse, Rat
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues around tyrosine 1526 of human integrin-beta4.
A431 and src-transformed Hct116 cells treated with pervanadate.
Liquid
Store at -20°C.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA
Concentration information loading...
Immunogen affinity purified
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to dephosphorylated integrin beta4 (Tyr-1526) peptide before affinity purification using phospho-integrin beta-4(Tyr-1526) peptide (without carrier).
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.
Polyclonal
IgG
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Western blot - Integrin beta 4 (phospho Y1526) antibody (ab29044)
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Our Abpromise guarantee covers the use of ab29044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/2000
WB: 1/1000Detects a band of approximately 202 kDa.
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
Membrane.
Target information above from: UniProt accessionP16144
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Integrin beta 4 (phospho Y1526) antibody (ab29044)

Western blot analysis of A431 (25 ug/lane) serum starved overnight (lane 1) and treated with pervanadate (1 mM) for 30 min (lanes 2-4). The blot was probed with rabbit polyclonal anti-phospho-integrin-beta4 (Tyr-1526). In the pervanadate-treated lanes, the polyclonal antibodies were used in the presence of no peptide (lane 2), phospho-integrin-beta4 (Tyr-1494) peptide (lane 3), or phospho-integrin-beta4 (Tyr-1526) peptide (lane 4).
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Western blot analysis of A431 (25 ug/lane) serum starved overnight (lane 1) and treated with pervanadate (1 mM) for 30 min (lanes 2-4). The blot was probed with rabbit polyclonal anti-phospho-integrin-beta4 (Tyr-1526). In the pervanadate-treated lanes, the polyclonal antibodies were used in the presence of no peptide (lane 2), phospho-integrin-beta4 (Tyr-1494) peptide (lane 3), or phospho-integrin-beta4 (Tyr-1526) peptide (lane 4).
0
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