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Western blot - Integrin beta 4 (phospho Y1526) antibody (ab29044)
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Product Name
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Integrin beta 4 (phospho Y1526) antibody
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See all Integrin beta 4 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Integrin beta 4 (phospho Y1526)
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Immunogen
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Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues around tyrosine 1526 of human integrin-beta4.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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The immunogen sequence used is found in all three integrin beta4 isoforms and is highly conserved in rat and mouse integrin beta4.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/2000.
WB: 1/1000. Detects a band of approximately 202 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 and src-transformed Hct116 cells treated with pervanadate.
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Cellular localization
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Cell Membrane; single pass type I membrane protein
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
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Relevance
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Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to dephosphorylated integrin beta4 (Tyr-1526) peptide before affinity purification using phospho-integrin beta-4(Tyr-1526) peptide (without carrier).
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Form
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Liquid
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Storage instructions
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Shipped at room temperature. Store at -20°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin beta 4 (phospho Y1526) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Integrin beta 4 (phospho Y1526) antibody images, references, products related to ab29044 and other tools.
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Integrin beta 4 (phospho Y1526) antibody images:
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 Western blot - Integrin beta 4 (phospho Y1526) antibody (ab29044)
Western blot analysis of A431 (25 ug/lane) serum starved overnight (lane 1) and treated with pervanadate (1 mM) for 30 min (lanes 2-4). The blot was probed with rabbit polyclonal anti-phospho-integrin-beta4 (Tyr-1526). In the pervanadate-treated lanes, the polyclonal antibodies were used in the presence of no peptide (lane 2), phospho-integrin-beta4 (Tyr-1494) peptide (lane 3), or phospho-integrin-beta4 (Tyr-1526) peptide (lane 4).
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References for Integrin beta 4 (phospho Y1526) antibody (ab29044)
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This product has been used in:
Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB; Human. PubMed: 18978815
If you publish research using ab29044 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Integrin beta 4
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Integrin beta 4 (phospho Y1526) antibody - more information
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