Anti-Integrin beta 5 antibody (ab15459)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 5 antibody (ab15459)

Human small intestine stained with anti-Integrin beta 5 antibody, ab15459 by Immunohistochemistry (FFPE-sections).

  Immunocytochemistry/ Immunofluorescence-Integrin beta 5 antibody(ab15459)

ICC/IF image of ab15459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 5 antibody (ab15459)

ab15459 staining Integrin beta 5 in Rat gastric epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 0.25% serum free protein for 20 minutes at 28°C. Samples were incubated with primary antibody (1/50 in antibody diluent) for 2 hours at 28°C. An FITC-conjugated Goat anti-IgG polyclonal (1/80) was used as the secondary antibody. Nuclei were counterstained with propidium iodide.

This image is courtesy of an Abreview submitted by Dr J. Chai

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