Biotin Anti-Integrin beta 7 antibody [FIB504] (ab95816)
Key features and details
- Biotin Rat monoclonal [FIB504] to Integrin beta 7
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Integrin beta 7 antibody [FIB504]
See all Integrin beta 7 primary antibodies -
Description
Biotin Rat monoclonal [FIB504] to Integrin beta 7 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus corresponding to Mouse Integrin beta 7. TK1 murine t-cell lymphoma
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Positive control
- Normal Human peripheral blood cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
FIB504 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use 0.125µg for 105 cells.
Range from 10^5 to 10^8 cells/test in a final volume of 100 µl. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
Use 0.125µg for 105 cells. Range from 10^5 to 10^8 cells/test in a final volume of 100 µl. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin. -
Tissue specificity
Expressed in a variety of leukocyte lines. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Domain
Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3695 Human
- Entrez Gene: 16421 Mouse
- Omim: 147559 Human
- SwissProt: P26010 Human
- SwissProt: P26011 Mouse
- Unigene: 654470 Human
- Unigene: 58 Mouse
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Alternative names
- Gut homing receptor beta subunit antibody
- Integrin beta 7 subunit antibody
- Integrin beta-7 antibody
see all
Images
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ICC/IF image of Integrin beta 7 staining on RAW264.7 cell line using ab95816 (1:300). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. The cells were incubated with ab95816 (1:300) for 1 hour at 20°C. The staining was detected using Streptavedin conjugated to Alexo Fluor 647 (1:350)
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Staining of normal Human peripheral blood cells with 0.06 µg of ab95816 followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
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Staining of normal Human peripheral blood cells with Anti-Human CD3 APC and 0.125 µg of Rat IgG2a ? Isotype Control Biotin. Cells in the lymphocyte gate were used for analysis.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab95816 has been referenced in 3 publications.
- Chai Z et al. Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPß Signaling Pathway in Cervical Cancer Cell Lines. Onco Targets Ther 12:10415-10425 (2019). PubMed: 31819523
- Meerschaert J et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). PubMed: 10570314
- Tidswell M et al. Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1. J Immunol 159:1497-505 (1997). PubMed: 9233649