![Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480) image](#ab52480_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Integrin linked ILK antibody [EP1593Y] (ab52480) image](#ab52480_2.jpg)
![Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480) image](#Integrin-linked-ILK-Primary-antibodies-ab52480-1.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-Integrin linked ILK antibody [EP1593Y]
See all Integrin linked ILK products (8) ...
Description
Rabbit monoclonal [EP1593Y] to Integrin linked ILK
Specificity
This antibody reacts with Integrin linked ILK
Tested applications
WB, IP, ICC, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, African Green Monkey
Immunogen
Synthetic peptide corresponding to residues near N terminus of human Integrin linked ILK
Positive control
Human heart muscle tissue and COS cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.05% BSA, 40% Glycerol, 0.09% Tris glycine
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1593Y
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2,000 - 1/10,000.Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP
IP: 1/30IP: 1/30
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
IHC-P: 1/50 - 1/100.(Antigen retrieval is strongly recommended.)
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding.
Post-translational
modifications
Autophosphorylated on serine residues.
Cellular localization
Cell junction > focal adhesion. Cell membrane.
Target information above from: UniProt accessionQ13418
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Database links
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all
Anti-Integrin linked ILK antibody [EP1593Y] images:
Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480)
![Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480)](/ps/datasheet/Images/52/ab52480/ab52480_1.jpg)
Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution + COS-1 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 51 kDa
Observed band size : 51 kDa
Immunohistochemistry (Paraffin-embedded sections) - Integrin linked ILK antibody [EP1593Y] (ab52480)
![Immunohistochemistry (Paraffin-embedded sections) - Integrin linked ILK antibody [EP1593Y] (ab52480)](/ps/datasheet/Images/52/ab52480/ab52480_2.jpg)
Immunohistochemical analysis of paraffin-embedded human heart muscle using ab52480 at a 1/50 dilution.
Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480)
![Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480)](/ps/datasheet/images/52/ab52480/Integrin-linked-ILK-Primary-antibodies-ab52480-1.jpg)
All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution
Lane 1 : Rat Brain
Lane 2 : Rat Heart
Lane 3 : Rat Kidney
Lane 4 : Rat Spleen
Predicted band size : 51 kDa
Exposure time : 3 minutes
Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480)
![Western blot - Integrin linked ILK antibody [EP1593Y] (ab52480)](/ps/datasheet/images/52/ab52480/Integrin-linked-ILK-Primary-antibodies-ab52480-2.jpg)
Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) + PC12
Predicted band size : 51 kDa
References for Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
This product has been referenced in:
- Lössner Det al. Integrin alphavbeta3 upregulates integrin-linked kinase expression in human ovarian cancer cells via enhancement of ILK gene transcription. J Cell Physiol 220:367-75 (2009).Read more (PubMed: 19334037) »
See 1 publication for this product
Publishing research using ab52480? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"