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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-Integrin linked ILK antibody
See all Integrin linked ILK products (8) ...
Description
Rabbit polyclonal to Integrin linked ILK
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to C terminal amino acids of Human Integrin linked ILK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1.0% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Western blot - Integrin linked ILK antibody (ab19025)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 - 20 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
Target
Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding.
Post-translational
modifications
Autophosphorylated on serine residues.
Cellular localization
Cell junction > focal adhesion. Cell membrane.
Target information above from: UniProt accessionQ13418
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Database links
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all
Anti-Integrin linked ILK antibody images:
Western blot - Integrin linked ILK antibody (ab19025)
Anti-Integrin linked ILK antibody (ab19025) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
References for Anti-Integrin linked ILK antibody (ab19025)
ab19025 has not yet been referenced specifically in any publications.
Publishing research using ab19025? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"