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Western blot - Integrin linked ILK antibody (ab2283)
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Immunocytochemistry/ Immunofluorescence - Integrin linked ILK antibody (ab2283)
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Product Name
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Integrin linked ILK antibody
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See all Integrin linked ILK antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Integrin linked ILK
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Immunogen
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Synthetic peptide derived from residues 1 - 100 of Mouse Integrin linked ILK.
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Chk, Gpig
(Due to sequence homology)
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Specificity
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This antibody recognises ILK (53kDa) in Western blots.
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml. ab2283 clearly stains the cytoplasm as expected. Foci within the nucleus are also seen. We have no explanation for this and we would appreciate any customer feedback.
WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Can be blocked with Integrin linked ILK peptide (ab13748).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin linked kinase (ILK), interacts with the cytoplasmic domain of beta 1 Integrin. ILK encodes a predicted 451 amino acid protein, with an apparent molecular weight of 59 kDa. The ILK protein is a serine/threonine protein kinase with 4 ankyrin like repeats. ILK regulates integrin mediated signal transduction.
The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.931 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Integrin linked ILK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Integrin linked ILK antibody images, references, products related to ab2283 and other tools.
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Integrin linked ILK antibody images:
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 Immunocytochemistry/ Immunofluorescence - Integrin linked ILK antibody (ab2283)
ICC/IF image of ab2283 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2283, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). ab2283 clearly stains the cytoplasm as expected for Integrin linked ILK. Some nuclear staining is also seen. We have no explanation for this nuclear staining and we would appreciate any customer feedback.
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References for Integrin linked ILK antibody (ab2283)
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This product has been used in:
Fielding AB et al. Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. J Cell Biol 180:681-9 (2008). ICC/IF; Human. PubMed: 18283114
If you publish research using ab2283 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Integrin linked ILK
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Integrin linked ILK antibody - more information
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