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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-Integrin linked ILK antibody
See all Integrin linked ILK products (8) ...
Description
Rabbit polyclonal to Integrin linked ILK
Specificity
This antibody recognises ILK (53kDa) in Western blots.
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Guinea pig
Immunogen
Synthetic peptide derived from residues 1 - 100 of Mouse Integrin linked ILK.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml. ab2283 clearly stains the cytoplasm as expected. Foci within the nucleus are also seen. We have no explanation for this and we would appreciate any customer feedback.
WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Can be blocked with Integrin linked ILK peptide (ab13748).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding.
Post-translational
modifications
Autophosphorylated on serine residues.
Cellular localization
Cell junction > focal adhesion. Cell membrane.
Target information above from: UniProt accessionQ13418
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Database links
- Entrez Gene: 374018 Chicken
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
see all
Anti-Integrin linked ILK antibody images:
Western blot - Integrin linked ILK antibody (ab2283)
All lanes : Anti-Integrin linked ILK antibody (ab2283) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Immunocytochemistry/ Immunofluorescence - Integrin linked ILK antibody (ab2283)
ICC/IF image of ab2283 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2283, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab2283 clearly stains the cytoplasm as expected for Integrin linked ILK. Some nuclear staining is also seen. We have no explanation for this nuclear staining and we would appreciate any customer feedback.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin linked ILK antibody (ab2283)
ab2283 staining Integrin linked ILK in mouse intestinal adenoma tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocking (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C was performed. The primary antibody was used diluted 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
This image is a courtesy of Antibody Solutions Ltd
References for Anti-Integrin linked ILK antibody (ab2283)
This product has been referenced in:
- Fielding ABet al. Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. J Cell Biol 180:681-9 (2008). ICC/IF; Human.Read more (PubMed: 18283114) »
See 1 publication for this product
Publishing research using ab2283? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"