Overview

  • Product name
    Anti-Interferon alpha 2b antibody [9D3]
    See all Interferon alpha 2b primary antibodies
  • Description
    Mouse monoclonal [9D3] to Interferon alpha 2b
  • Host species
    Mouse
  • Tested applications
    Suitable for: Sandwich ELISA, Inhibition Assay, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Interferon alpha 2b.
    Database link: P01563

  • Positive control
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR314306-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    9D3
  • Myeloma
    NS0/1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

This antibody can be used in a sandwich ELISA for the determination of human IFN-a2b. It recognizes different epitopes than anti-Interferon alpha 2b antibody [4E10] (HRP) (ab5258). We recommend using this antibody as a capture antibody and ab5258 as a detection antibody.
Inhibition Assay 1/400 - 1/1000.
WB 1/1000 - 1/5000.

At 5 µg/ml, this antibody inhibits 90% of IFN-a2b antiviral activity in vitro: The bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Interferon was pre-incubated with the ab9386 for 2 hours at RT and then added to the cell monolayer. 24 hours later the cells were infected with EMCV and cultured for further 24 hours. Antiviral activity of IFN was evaluated using crystal-violet staining. PAbs to respective interferons and an irrelevant MAb of same isotype were used as controls.
Indirect ELISA 1/1000 - 1/10000.

Target

  • Function
    Produced by macrophages, IFN-alpha have antiviral activities.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Alpha 2a interferon antibody
      • IFN alpha 2b antibody
      • IFN-alpha-2 antibody
      • IFNA antibody
      • Ifna2 antibody
      • IFNA2_HUMAN antibody
      • INFA2 antibody
      • Interferon alpha 2 antibody
      • Interferon alpha A antibody
      • Interferon alpha-2 antibody
      • Interferon alpha-A antibody
      • LeIF A antibody
      • LeIFA antibody
      see all

    Images

    • Western blot - Anti-Interferon alpha 2b antibody [9D3] (ab9386)
      Western blot - Anti-Interferon alpha 2b antibody [9D3] (ab9386)Landowski et al. PLoS One. 2015 Aug 26;10(8):e0134723. doi: 10.1371/journal.pone.0134723. eCollection 2015.fig 11

      The nitrocellulose membrane was blocked for 1 hour at room temperature with 5% milk in TBST buffer. The individual blots were probed  to detect the appropriate model protein for 1 hour at room temperature on a shaker. 

      ab9386 was used at 1 μg/ml diluted in TBST. After briefly washing the blot membranes with TBST, the secondary antibody was added for 1 hour at room temperature with shaking. The secondary goat anti-mouse AP conjugated antibody was diluted 1:10,000 in TBST.

      For full details see figure 11.

    • ELISA - Anti-Interferon alpha 2b antibody [9D3] (ab9386)
      ELISA - Anti-Interferon alpha 2b antibody [9D3] (ab9386)

      Standard curve for the determination of human interferon-alpha 2b by two-epitope ELISA: capture antibody 9D3 (ab9386), peroxidase-labeled antibody 4E10 (ab9388).

    • Neutralising - Anti-Interferon alpha 2b antibody [9D3] (ab9386)
      Neutralising - Anti-Interferon alpha 2b antibody [9D3] (ab9386)

      Neutralizing activity of monoclonal antibody 9D3 in vitro. Interferon antiviral bioassay: L41 cells, mouse encephalomyacarditis virus (EMCV). Antibodies at 5 µg/ml. Control 1 - without EMCV (non-infected cells), control 2 - without interferon, control 3 - irrelevant antibody.

    References

    This product has been referenced in:
    • Landowski CP  et al. Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei. Microb Cell Fact 15:104 (2016). Read more (PubMed: 27287473) »
    • Chen J  et al. Dissolving microneedle-based intradermal delivery of interferon-a-2b. Drug Dev Ind Pharm 42:890-6 (2016). Read more (PubMed: 26467418) »

    See all 8 Publications for this product

    Publishing research using ab9386? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    6 reviews or Q&A

    10
    Votes: Highest First

    We would be interested in knowing how do you quantify your antibodies, especially for cat. no ab9386 and ab9388. Thanks in advance!

    Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hop...

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    I would like to know the quantity that will be provided in each tube lot after lot? I understand it’s 100 ul but would it be 200ug for each lot? Thanks in advance

    Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a m...

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    I'm interested in using this antibody for an inhibition assay, but I wanted to know how many units of interferon alpha 2b can be blocked with the antibody.

    Thank you for your inquiry. Neutralizing activity of ab9386 has been tested in cell line-based bioassay where IFN has been applied in a concentration range between 4 IU/ml and 0.125 IU/ml. If you use lower concentrations of IFN (below 0.125 IU/ml...

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    BATCH NUMBER 16494 ORDER NUMBER 114870 DESCRIPTION OF THE PROBLEM No signal SAMPLE pure human IFN alpha PRIMARY ANTIBODY 1st set of experiments: PcAb anti IFN alpha from [competitor A] (raised in rabbit) 2nd set of experi...

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    I'm sorry to hear you are having a problem with ab9386. The use of monoclonal antibodies in sandwich ELISA always requires a careful selection of a suitable pair of antibodies. Antibodies should be complementary, e.g. they should recognize different...

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    Re. Mouse monoclonal (4E10) to Interferon alpha 2b & Mouse monoclonal (9D3) to human interferon alpha 2b I would like to use these two anitbodies together in ELISA. Can you send me a protocol please?

    To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coat...

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    I would like to know if this antibody recognises a single epitope on IFN alpha 2b or more then one ? Many thanks in advance.

    Usually, each monoclonal antibody recognises only a single epitope on the antigen because each hybridoma clone is derived from a single B-cell. This antibody reacts well with both native and SDS-denaturated IFN-alpha 2b. Therefore, we suppose that the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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