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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab9386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.
This antibody can be used in a sandwich ELISA for the determination of human IFN-a2b. It recognizes different epitopes than Anti-Interferon alpha 2b antibody [4E10] (HRP) (ab5258). We recommend using this antibody as a capture antibody and ab5258 as a detection antibody.
|Inhibition Assay||1/400 - 1/1000.|
|WB||1/1000 - 1/5000. At 5 µg/ml, this ab inhibits 90% of IFN-a2b antiviral activity in vitro: The bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24h later the cells were infected with EMCV and cultured for further 24h. Antiviral activity of IFN was evaluated using crystal-violet staining. PAbs to respective interferons and an irrelevant MAb of same isotype were used as controls.|
|Indirect ELISA||1/1000 - 1/10000.|
Standard curve for the determination of human interferon-alpha 2b by two-epitope ELISA: capture antibody 9D3 (ab9386), peroxidase-labeled antibody 4E10 (ab9388).
Neutralizing activity of monoclonal antibody 9D3 in vitro. Interferon antiviral bioassay: L41 cells, mouse encephalomyacarditis virus (EMCV). Antibodies at 5