Anti-Interferon alpha antibody [ST29] (ab20200)
Overview
- Product nameAnti-Interferon alpha antibody [ST29]See all Interferon alpha primary antibodies ...
- DescriptionMouse monoclonal [ST29] to Interferon alpha
- Tested applicationsSandwich ELISA, Neutralising more details
- Species reactivityReacts with: Human
- Immunogen
Human interferon (HuIFN) produced by Namalwa cells (HuIFN-aN).
- General notesMyeloma: NS2
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... - PurityIgG fraction
- Clonality Monoclonal
- Clone numberST29
- IsotypeIgG1
- Light chain typeunknown
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab20200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab84257 as Detection. |
| Neutralising | Neut: Use at an assay dependent dilution. |
Target
- FunctionProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
- Sequence similaritiesBelongs to the alpha/beta interferon family.
- Cellular localizationSecreted.
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Database links
- Entrez Gene: 3439 Human
- Entrez Gene: 3447 Human
- Omim: 147660 Human
- SwissProt: P01562 Human
- Unigene: 37026 Human
- Unigene: 533471 Human
Target information above from: UniProt accession
P01562
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- IFL antibodyIFN alpha antibodyIFN antibody
- IFN-alpha-1/13 antibodyIFNA@ antibodyIFNA1 antibodyIFNA1_HUMAN antibodyIFNA13 antibodyInterferon alpha 1 antibodyInterferon alpha 1/13 antibodyInterferon alpha 13 antibodyInterferon alpha D antibodyInterferon alpha leukocyte antibodyInterferon alpha-1/13 antibodyInterferon alpha-D antibodyInterferon leukocyte antibodyLeIF D antibodyMGC138207 antibodyMGC138505 antibodyMGC138507 antibody
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Anti-Interferon alpha antibody [ST29] images
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![Sandwich ELISA - Interferon alpha antibody [ST29] (ab20200)](http://a.abcam.com/ps/datasheet/images/20/ab20200/Interferon-alpha-Primary-antibodies-ab20200-3.jpg)
Sandwich ELISA - Interferon alpha antibody [ST29] (ab20200)Standard Curve for Interferon alpha (Analyte: ab48750) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [ST29] to Interferon alpha (ab20200) at 5ug/ml and Detector Antibody Rabbit polyclonal to Interferon alpha (Biotin) (ab84257) at 0.5ug/ml.
Protocols
References for Anti-Interferon alpha antibody [ST29] (ab20200)
This product has been referenced in:
- Shearer M et al. Monoclonal antibodies that distinguish between subspecies of human interferon-alpha and that detect interferon oligomers. J Immunol 133:3096-101 (1984). Read more (PubMed: 6491281) »
