Anti-Interferon gamma antibody [B27] (Biotin) (ab24950)

Overview

  • Product nameAnti-Interferon gamma antibody [B27] (Biotin)See all Interferon gamma primary antibodies ...
  • Description
    Mouse monoclonal [B27] to Interferon gamma (Biotin)
  • ConjugationBiotin
  • Specificityab24950 does not bind to denatured Interferon gamma.
  • Tested applicationsNeutralising, ELISA, Flow Cyt, ICC/IF, IP more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein: E. coli expressed recombinant human interferon gamma.

Properties

Applications

Our Abpromise guarantee covers the use of ab24950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Neutralising
ELISA
Flow Cyt
ICC/IF
IP
  • Application notesELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    Neut: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
    • Tissue specificityReleased primarily from activated T lymphocytes.
    • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
    • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
    • Post-translational
      modifications
      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
    • Cellular localizationSecreted.
    • Target information above from: UniProt accession P01579 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • IFG antibody
      • IFI antibody
      • IFN Gamma antibody
      • IFN Immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune Interferon antibody
      • Interferon gamma antibody
      • Interferon Gamma Precursor antibody
      • Macrophage Activating Factor antibody
      • MAF antibody
      • T Cell Interferon antibody
      • Type II Interferon antibody
      see all

    References for Anti-Interferon gamma antibody [B27] (Biotin) (ab24950)

    This product has been referenced in:
    • Amara RR  et al. Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine. Science 292:69-74 (2001). Read more (PubMed: 11393868) »
    • Abrams JS  et al. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127:5-24 (1992). Read more (PubMed: 1387110) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting Abcam regarding ab24950.


    Regarding the expiration of this product, we expect the product to be stable for up to 1 year when stored at 4deg C.


    For storage we do not recommend further diluting the ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"