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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab9801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/1000 - 1/10000.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493Used as the detection antibody in conjuction with recommended pair. sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells.|
|Inhibition Assay||Use at an assay dependent dilution. Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls.|
|WB||1/500 - 1/1000. Detects a band of approximately 16-21 kDa.|