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Overview

  • Product nameAnti-Interferon gamma antibody [XMG1.2] (FITC)See all Interferon gamma primary antibodies ...
  • Description
    Rat monoclonal [XMG1.2] to Interferon gamma (FITC)
  • ConjugationFITC
  • Tested applicationsICC/IF, Flow Cyt, ELISA more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length Mouse protein expressed in E. coli

Properties

Applications

Our Abpromise guarantee covers the use of ab87030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent dilution.
Flow Cyt Flow Cyt: Use at an assay dependent dilution.
ELISA ELISA: Use at an assay dependent dilution. Ab87303 can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-? protein levels. Biotinylated form of the antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-? as the standard.

Target

  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01579 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        IFG antibodyIFI antibodyIFN gamma antibody
        IFN-gamma antibodyIFNG antibodyIFNG antibodyIFNG_HUMAN antibodyImmune interferon antibodyInterferon gamma antibody
      see all

    References for Anti-Interferon gamma antibody [XMG1.2] (FITC) (ab87030)

    This product has been referenced in:
    • Vikingsson A  et al. Altered kinetics of CD4+ T cell proliferation and interferon-gamma production in the absence of CD8+ T lymphocytes in virus-infected beta2-microglobulin-deficient mice. Cell Immunol 173:261-8 (1996). Read more (PubMed: 8912885) »
    • Hsieh B  et al. In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells. J Immunol 156:232-7 (1996). Read more (PubMed: 8598467) »

    See all 3 Publications for this product

    Publishing research using ab87030 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"