Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal to Interferon gamma (ab9658).

To detect IFN-gamma by sandwich ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma.

Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIFN-gamma (50 pg/ml), a concentration of 0.04 - 0.06 ng/ml of this antibody is required.
ICC/IF 1/50.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml.

Target

  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

Anti-Interferon gamma antibody images

  • ab9657 staining Interferon gamma in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen
  • To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a compatible secondary detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.

References for Anti-Interferon gamma antibody (ab9657)

This product has been referenced in:
  • Lood C  et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nat Med N/A:N/A (2016). Neutralising . Read more (PubMed: 26779811) »
  • Safronetz D  et al. Pathophysiology of hantavirus pulmonary syndrome in rhesus macaques. Proc Natl Acad Sci U S A 111:7114-9 (2014). IHC (PFA fixed) ; Macaque Monkey . Read more (PubMed: 24778254) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Both Tris-EDTA and Citrate-Sodium
Permeabilization No
Specification Lymph node
Blocking step (agent) for 15 minute(s) · Concentration: 0.25% · Temperature: 22°C
Fixative Formaldehyde
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Submitted Feb 02 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human tumor lymph node (non-Hodgkin lymphoma, T ce)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0, 15 min
Permeabilization No
Specification Human tumor lymph node (non-Hodgkin lymphoma, T ce
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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Submitted Oct 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
Sample Human Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde
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Submitted Dec 17 2014

Application Western blot
Sample Mouse Serum (Mouse Serum 0.3 ul)
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Loading amount 3 µg
Specification Mouse Serum 0.3 ul
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Submitted Jul 11 2014

All of these antibodies recognise cytokines which are present in inflammed tissue therefore any inflammed tissue would be a good positive control.

In our catalogue, we have several cancer slides which would be appropriate:
tonsil: (ab514...

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Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (mammary gland lymph node)
Specification mammary gland lymph node
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Dr. Lynn Dong

Verified customer

Submitted Mar 02 2012

A good alternative to tonsil is spleen and also tissue from the digestive tract, for instance colon or small intestine.

Thank you for your enquiry. This antibody is sold at 100 ug per vial. We woudl recommend to reconsitute this in 200 ul of sterile distilled water. This will provide a stock concentration of 0.5 mg/ml. With regards to isotype, this antibody is a polyclo...

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Thank you for your enquiry. As far as we know only the pH 6 citrate buffer has been tested as a retrieval buffer for use with ab9657. It is certainly possible that a different pH buffer will also work, however we do not have any experimental data to...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Recombinant human IFN-gamma)
Loading amount 3 µg
Specification Recombinant human IFN-gamma
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"