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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Overview
Product name
Anti-Intra Acrosomal Protein antibody [Ds-2]
See all Intra Acrosomal Protein products (6) ...
Description
Mouse monoclonal [Ds-2] to Intra Acrosomal Protein
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Dog
Immunogen
Tissue / cell preparation (Dog). Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15 mM sodium azide
Constituents: Phosphate buffered saline, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Primary antibody notes
The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of canine sperms.
Clonality
Monoclonal
Clone number
Ds-2
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell differentiation
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC
Application notes
ICC: Use at an assay dependent dilution, membrane permeabilization (acetone) essential.
WB: Use at an assay dependent dilution. Predicted molecular weight: 32 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.
Cellular localization
Acrosome. Note= Nascent acrosomal vesicle of Golgi phase spermatids.
Alternative names
- D11S4365 antibody
- Acrosomal protein SP 10 antibody
- acrosomal vesicle protein 1 antibody
see all
References for Anti-Intra Acrosomal Protein antibody [Ds-2] (ab14300)
ab14300 has not yet been referenced specifically in any publications.
Publishing research using ab14300? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"