Anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) (ab77238)
Overview
- Product nameAnti-Intra Acrosomal Protein antibody [Hs-14] (FITC)See all Intra Acrosomal Protein primary antibodies ...
- DescriptionMouse monoclonal [Hs-14] to Intra Acrosomal Protein (FITC)
- ConjugationFITC
- Conjugation notesThe purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
- Tested applicationsFlow Cyt more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Freshly ejaculated human sperms (washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization).
- Positive controlSperm cells.
Properties
- FormLiquid
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.2% BSA(high-grade protease free), Tris buffered saline -
Concentration information loading... - PuritySize exclusion
- Clonality Monoclonal
- Clone numberHs-14
- IsotypeIgM
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- Tissue specificityTestis.
- Cellular localizationCytoplasmic vesicle > secretory vesicle > acrosome. Nascent acrosomal vesicle of Golgi phase spermatids.
-
Database links
- Entrez Gene: 56 Human
- Entrez Gene: 56 Human
- Entrez Gene: 11451 Mouse
- Omim: 102525 Human
- SwissProt: P26436 Human
- SwissProt: P26436 Human
- SwissProt: P50289 Mouse
- Unigene: 169222 Human
- Unigene: 596575 Human
- Unigene: 4776 Mouse
see all
Target information above from: UniProt accession
P26436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Acrosomal protein SP 10 antibodyAcrosomal protein SP-10 antibodyAcrosomal vesicle protein 1 antibody
- ACRV1 antibodyASPX_HUMAN antibodyD11S4365 antibodyMsa63 antibodySP 10 antibodySp10 antibodySPACA2 antibodySperm protein 10 antibody
see all
References for Anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) (ab77238)
This product has been referenced in:
- Peknicova J et al. Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol 53:42-9 (2005). Read more (PubMed: 15667524) »
- Chládek D et al. [Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization] Ceska Gynekol 65:28-32 (2000). Read more (PubMed: 10750294) »
