Intracellular GSH Assay Kit (ab112132)
- Product nameIntracellular GSH Assay KitSee all Glutathione kits ...
- Tests1 x 100 test
- Sample typeAdherent cells, Suspension cells
- Product overview
There are a variety of parameters that can be used for monitoring cell apoptosis. ab112132 is designed to detect cell apoptosis by measuring the decrease in reduced glutathione (GSH). GSH is important for maintaining redox level of cells. It is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cells.
ab112132 Intracellular GSH Assay Kit uses our proprietary non-fluorescent Green Dye, which becomes strongly fluorescent upon reacting with thiol (including GSH in cells). In normal cells, the Green Dye is accumulated primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Green Dye staining intensity is decreased. Cells stained with Green Dye can be visualized with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel).
ab112132 can be used together with other reagents, such as 7-AAD for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.
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- Tested applicationsFlow Cyt more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Assay Buffer 1 x 100ml DMSO 1 x 500µl Thiol Green Indicator 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab112132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Intracellular GSH Assay Kit images
The decrease in the fluorescence intensity of ab112132 Green Dye with the addition of Camptothecin in Jurkat cells. Jurkat cells were treated overnight without (blue line) or with 20 µM camptothecin (pink line) in a 37 oC, 5% CO2 incubator, and then dye loaded with ab112132 Green Dye for 30 minutes. The fluorescence intensity of Green Dye was measured with a flow cytometer using the FL1 channel.
References for Intracellular GSH Assay Kit (ab112132)
ab112132 has not yet been referenced specifically in any publications.