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Intracellular GSH Assay Kit (ab112132)

Q&A (1)

Overview

  • Product nameIntracellular GSH Assay KitSee all Glutathione kits ...
  • Tests
    1 x 100 test
  • Sample type
    Adherent cells, Suspension cells
  • Product overview

    There are a variety of parameters that can be used for monitoring cell apoptosis. ab112132 is designed to detect cell apoptosis by measuring the decrease in reduced glutathione (GSH). GSH is important for maintaining redox level of cells. It is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cells.

    ab112132 Intracellular GSH Assay Kit uses our proprietary non-fluorescent Green Dye, which becomes strongly fluorescent upon reacting with thiol (including GSH in cells). In normal cells, the Green Dye is accumulated primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Green Dye staining intensity is decreased. Cells stained with Green Dye can be visualized with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel).

    ab112132 can be used together with other reagents, such as 7-AAD for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFlow Cyt more details

Properties

  • RelevanceGlutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis.
    • Alternative names
        GSHGSSG

    Applications

    Our Abpromise guarantee covers the use of ab112132 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt Flow Cyt

    Intracellular GSH Assay Kit images

    • Flow Cytometry - Intracellular GSH Assay Kit (ab112132)
      Flow Cytometry - Intracellular GSH Assay Kit (ab112132)
      The decrease in the fluorescence intensity of ab112132 Green Dye with the addition of Camptothecin in Jurkat cells. Jurkat cells were treated overnight without (blue line) or with 20 µM camptothecin (pink line) in a 37 oC, 5% CO2 incubator, and then dye loaded with ab112132 Green Dye for 30 minutes. The fluorescence intensity of Green Dye was measured with a flow cytometer using the FL1 channel.

    Protocols

    References for Intracellular GSH Assay Kit (ab112132)

    ab112132 has not yet been referenced specifically in any publications.

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    Hi,

    I am using Greiner Thincert Culture Inserts for 6-well plates and A549 cells in my exposure experiments. The goal is to expose the cells at an air/liquid interphase for a certain period and to measure cell viability, oxidative stress marker...

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    Thank you for your enquiry and interest in our products.

    MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the cells...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"