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Western blot - Invadolysin antibody (ab58997)
(enlarge)
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Product Name
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Invadolysin antibody
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See all Invadolysin antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Invadolysin
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Immunogen
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Synthetic peptide based on the catalytic domain of human Invadolysin.
Immunizing peptide available on request, please contact a customer service representative to ask for availability.
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Reacts with
(species key)
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Hu
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Specificity
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ab58997 recognizes all but the shortest form of human Invadolysin.
We have a range of domain specific antibodies for this target. For a full list please see all Invadolysin antibodies.
We have a range of domain specific antibodies for this target. For a full list please see all Invadolysin antibodies
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 - 1/5000 dependent on substrate. Predicted molecular weight: 74 kDa.
A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
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Relevance
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Invadolysin, also known as Leismanolysin-Like proteinase, is a metalloproteinase of the MA clan, family M8, which is distinguished from the other families of metalloproteinases by an insert in the zinc binding site. The “classical” zinc binding motif of HExxHxxxxH contains an insert of 65 residues in the Leishmania form of Leishmanolysin, and varies from 62-105 residues amongst different parasite leishmanolysin isoforms.
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Invadolysin antibody (ab58997) |
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 89782 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Invadolysin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Invadolysin antibody images, references, products related to ab58997 and other tools.
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Invadolysin antibody images:
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 Western blot - Invadolysin antibody (ab58997)
All lanes : Invadolysin antibody (ab58997) at 1/1000 dilution
Lane 1 : Lysates of A-431 cells (epidermoid carcinoma - skin) treated with SF
Lane 2 : Lysates of A-431 cells (epidermoid carcinoma - skin) treated with RA
Lane 3 : Lysates of A-431 cells (epidermoid carcinoma - skin) treated with TPA
Predicted band size : 74 kDa
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Search PubMed (MEDLINE) for references to Invadolysin
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Invadolysin antibody - more information
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Customer reviews (feedback) regarding Invadolysin antibody |
Customer FAQs regarding Invadolysin antibody |
Protocols for Invadolysin antibody |
Price and availability of products related to Invadolysin antibody |
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Invadolysin antibody - related products:
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Invadolysin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Invadolysin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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