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Products:Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Overview
Product name
Anti-Invadolysin antibody
See all Invadolysin products (4) ...
Description
Rabbit polyclonal to Invadolysin
Specificity
ab59001 recognizes all of the published forms of human Invadolysin. We have a range of domain specific antibodies for this target. For a full list please see all Invadolysin antibodies.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the cysteine-rich region following the catalytic domain of human Invadolysin. Immunizing peptide available on request, please contact a customer service representative to ask for availability.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
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Western blot - Invadolysin antibody (ab59001)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 - 1/5000 dependent on substrate. Predicted molecular weight: 74 kDa.
A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Invadolysin, also known as Leismanolysin-Like proteinase, is a metalloproteinase of the MA clan, family M8, which is distinguished from the other families of metalloproteinases by an insert in the zinc binding site. The “classical” zinc binding motif of HExxHxxxxH contains an insert of 65 residues in the Leishmania form of Leishmanolysin, and varies from 62-105 residues amongst different parasite leishmanolysin isoforms.
Cellular localization
Cytoplasmic
Alternative names
- Gp63 antibody
- GP63 like antibody
- Leishmanolysin Like Peptidase antibody
see all
Database links
- Entrez Gene: 89782 Human
- SwissProt: Q96KR4 Human
Anti-Invadolysin antibody images:
Western blot - Invadolysin antibody (ab59001)
All lanes : Anti-Invadolysin antibody (ab59001) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with SF)
Lane 2 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
Lane 3 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with IL1-beta)
Lysates/proteins at 20 µl per lane.
Predicted band size : 74 kDa
References for Anti-Invadolysin antibody (ab59001)
ab59001 has not yet been referenced specifically in any publications.
Publishing research using ab59001? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"