Overview
- Product nameAnti-Involucrin antibody [SY5] (Biotin)See all Involucrin primary antibodies ...
- DescriptionMouse monoclonal [SY5] to Involucrin (Biotin)
- ConjugationBiotin
- Tested applicationsWB, IP, ELISA, IHC-P, ICC/IF more details
- Species reactivityReacts with: Rat, Dog, Human, Pig, Monkey, Gorilla
- Immunogen
Human keratinocyte Involucrin
- EpitopeCodon 421-568.
- Positive controlLS174T cell lysate; Skin tissue.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityProtein G purified
- Purification notesab79031 is purified from ascites fluid by Protein G chromatography.
- Clonality Monoclonal
- Clone numberSY5
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab79031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IP | |
| ELISA | |
| IHC-P | |
| ICC/IF |
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37°C or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
IP: Use at 2µg/mg of lysate (denatured).
WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 68 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
- Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
- Sequence similaritiesBelongs to the involucrin family.
- Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. - Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
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Database links
- Entrez Gene: 609423 Dog
- Entrez Gene: 3713 Human
- Entrez Gene: 407242 Pig
- Entrez Gene: 407242 Pig
- Entrez Gene: 60583 Rat
- Omim: 147360 Human
- SwissProt: P18174 Dog
- SwissProt: P07476 Human
- SwissProt: P18175 Pig
- SwissProt: P48998 Rat
- Unigene: 516439 Human
- Unigene: 142591 Rat
see all
Target information above from: UniProt accession
P07476
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- INVO_HUMAN antibodyinvolucrin antibodyivl antibody
Anti-Involucrin antibody [SY5] (Biotin) images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Involucrin antibody [SY5] (Biotin) (ab79031)](http://a.abcam.com/ps/datasheet/images/79/ab79031/Involucrin-Primary-antibodies-ab79031-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Involucrin antibody [SY5] (Biotin) (ab79031)ab79031 at 2µg/ml staining Involucrin in human skin by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining in the epidermal region. The purified form of this antibody was used for staining. -
![Western blot - Involucrin antibody [SY5] (Biotin) (ab79031)](http://a.abcam.com/ps/datasheet/images/79/ab79031/Involucrin-Primary-antibodies-ab79031-3.jpg)
Western blot - Involucrin antibody [SY5] (Biotin) (ab79031)anti-Involucrin at 2 µg/ml + LS174T cell lysate
Predicted band size : 68 kDa
References for Anti-Involucrin antibody [SY5] (Biotin) (ab79031)
ab79031 has not yet been referenced specifically in any publications.
